LOCUS       Exported                5771 bp ds-DNA     circular SYN 17-DEC-2018
DEFINITION  Eukaryotic expression vector pCR3.1mBCL-XL, complete sequence.
ACCESSION   AF060226
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5771)
  AUTHORS   Pirtskhalaishvili G, Shurin GV, Gambotto A, Esche C, Wahl M, 
            Yurkovetsky ZR, Robbins PD, Shurin MR.
  TITLE     Transduction of dendritic cells with Bcl-xL increases their 
            resistance to prostate cancer-induced apoptosis and antitumor effect
            in mice
  JOURNAL   J. Immunol. 165 (4), 1956-1964 (2000)
  PUBMED    10925278
REFERENCE   2  (bases 1 to 5771)
  AUTHORS   Gambotto A, Pagliano O, Shurin M, Robbins PD.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-APR-1998) Vector Core Facility, University of 
            Pittsburgh, 300 Technology Drive, Pittsburgh, PA 15219, USA
REFERENCE   3  (bases 1 to 5771)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..5771
                     /organism="Eukaryotic expression vector pCR3.1mBCL-XL"
                     /mol_type="genomic DNA"
                     /db_xref="taxon:75965"
     regulatory      1..596
                     /regulatory_class="promoter"
                     /note="CMV"
     enhancer        10..389
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        390..593
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     regulatory      638..657
                     /regulatory_class="promoter"
                     /note="T7; priming site also"
     promoter        638..656
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             747..1448
                     /codon_start=1
                     /product="murine BCL-XL"
                     /label=murine BCL-XL
                     /note="BALB/c form"
                     /protein_id="AAC15799.1"
                     /translation="MSQSNRELVVDFLSYKLSQKGYSWSQFSDVEENRTEAPEETEAER
                     ETPSAINGNPSWHLADSPAVNGATGHSSSLDAREVIPMAAVKQALREAGDEFELRYRRA
                     FSDLTSQLHITPGTAYQSFEQVVNELFRDGVNWGRIVAFFSFGGALCVESVDKEMQVLV
                     SRIASWMATYLNDHLEPWIQENGGWDTFVDLYGNNAAAESRKGQERFNRWFLTGMTVAG
                     VVLLGSLFSRK"
     misc_feature    1524..1541
                     /label=pCR3.1 reverse priming site
                     /note="pCR3.1 reverse priming site"
     polyA_signal    1530..1754
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      1827..2500
                     /label=ColE1
                     /note="ColE1"
     rep_origin      complement(1886..2474)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     polyA_signal    2803..2850
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     CDS             complement(3082..3870)
                     /codon_start=1
                     /product="neomycin/kanamycin resistance protein"
                     /label=neomycin/kanamycin resistance protein
                     /protein_id="AAC15800.1"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDQEHQGL
                     APAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIALA
                     TRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     promoter        complement(3905..4262)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      3905..4243
                     /label=SV40 promoter and origin
                     /note="SV40 promoter and origin"
     rep_origin      3919..4054
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             complement(4322..5182)
                     /codon_start=1
                     /product="ampicillin resistance protein"
                     /label=ampicillin resistance protein
                     /protein_id="AAC15801.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(5183..5287)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      5313..5769
                     /label=f1
                     /note="f1"
     rep_origin      5314..5769
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"