LOCUS       Exported                5162 bp ds-DNA     circular SYN 17-DEC-2018
DEFINITION  Shuttle vector pCR-Zeo, complete sequence.
ACCESSION   MF287366
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5162)
  AUTHORS   Yan MY, Yan HQ, Ren GX, Zhao JP, Guo XP, Sun YC.
  TITLE     CRISPR-Cas12a-assisted recombineering in bacteria
  JOURNAL   Appl. Environ. Microbiol. (2017) In press
  PUBMED    28646112
REFERENCE   2  (bases 1 to 5162)
  AUTHORS   Yan M-Y., Yan H-Q., Ren G-X., Zhao J-P., Guo X-P., Sun Y-C.
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUN-2017) Moh Key Laboratory of Systems Biology of 
            Pathogens, Chinese Academy of Medical Sciences and Peking Union 
            Medical College Institute of Pathogen Biology, 9, Dongdan Santiao, 
            Dongcheng District, Beijing 100730, China
REFERENCE   3  (bases 1 to 5162)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5162
                     /organism="Shuttle vector pCR-Zeo"
                     /mol_type="other DNA"
                     /db_xref="taxon:2018567"
     regulatory      381..5154
                     /regulatory_class="promoter"
                     /note="hsp60 promoter"
     terminator      564..610
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     regulatory      747..903
                     /regulatory_class="promoter"
                     /note="pHsp70"
     CDS             904..1278
                     /codon_start=1
                     /gene="BleoR"
                     /product="BleoR"
                     /label=BleoR
                     /note="antibiotic-binding protein; Zeocin resistance"
                     /protein_id="ASK86100.1"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     gene            904..1278
                     /gene="BleoR"
                     /label=BleoR
     rep_origin      1465..4218
                     /gene="PAL5000ts"
                     /label=oriM
                     /note="oriM"
     gene            1465..4218
                     /gene="PAL5000ts"
                     /label=PAL5000ts
     rep_origin      4357..4945
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"