LOCUS       Exported                3012 bp ds-DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector pCPT8, complete sequence.
ACCESSION   AF092546
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3012)
  AUTHORS   Pitulle C, Pace NR.
  TITLE     Novel T-cloning vector for plasmid-based 16S rDNA analysis
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3012)
  AUTHORS   Pitulle C.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-1998) Plant & Microbial Biology, University of 
            California, 371 Koshland Hall, Berkeley, CA 94720-3102, USA
REFERENCE   3  (bases 1 to 3012)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..3012
                     /organism="Cloning vector pCPT8"
                     /mol_type="genomic DNA"
                     /db_xref="taxon:88575"
     misc_feature    1..136
                     /label=multiple cloning site
                     /note="multiple cloning site"
     misc_feature    1
                     /label=T7 RNA polymerase initiation site
                     /note="T7 RNA polymerase initiation site"
     misc_feature    15..29
                     /label=XcmI restriction site
                     /note="XcmI restriction site"
     misc_feature    32..46
                     /label=XcmI restriction site
                     /note="XcmI restriction site"
     promoter        complement(134..152)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     misc_feature    complement(136)
                     /label=SP6 RNA polymerase initiation site
                     /note="SP6 RNA polymerase initiation site"
     primer_bind     complement(170..186)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    194..210
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(218..248)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    263..284
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(572..1160)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(1331..2191)
                     /codon_start=1
                     /product="beta-lactamase"
                     /label=beta-lactamase
                     /protein_id="AAD16103.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCHTLLSRIDAGQEQLGRRARYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMPTTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(2192..2296)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(2377..2832)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     2973..2989
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        2996..2
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"