LOCUS       Exported                6479 bp ds-DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector pCPP5312, complete sequence.
ACCESSION   EU024545
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6479)
  AUTHORS   Kvitko BH, Ramos AR, Morello JE, Oh HS, Collmer A.
  TITLE     Identification of harpins in Pseudomonas syringae pv. tomato DC3000,
            which are functionally similar to HrpK1 in promoting translocation 
            of type III secretion system effectors
  JOURNAL   J. Bacteriol. 189 (22), 8059-8072 (2007)
  PUBMED    17873033
REFERENCE   2  (bases 1 to 6479)
  AUTHORS   Kvitko BH, Ramos AR, Morello JE, Oh H-S., Collmer A.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JUL-2007) Plant Pathology, Cornell University, 334 
            Plant Science, Ithaca, NY 14853, USA
REFERENCE   3  (bases 1 to 6479)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..6479
                     /organism="Cloning vector pCPP5312"
                     /mol_type="other DNA"
                     /db_xref="taxon:458222"
     promoter        complement(15..43)
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     protein_bind    210..234
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_recomb     211..231
                     /label=attB1
                     /note="attB1"
     regulatory      353..385
                     /regulatory_class="promoter"
                     /note="hrp box"
     CDS             420..2177
                     /codon_start=1
                     /product="AvrPto1-Cya fusion protein"
                     /label=AvrPto1-Cya fusion protein
                     /note="AvrPto1 from P. syringae pv.tomato DC3000 fused to 
                     the catalytic domain of calmodulin-dependant adenylate 
                     cyclase from Bordatella pertussis"
                     /protein_id="ABS87317.1"
                     /translation="MGNICVGGSRMAHQVNSPDRVSNNSGDEDNVTSSQLLSVRHQLAE
                     SAGVPRDQHEFVSNQAPQSLRNRYNNLYSHTQRTLDMADMQHRYMTGASGINPGMLPHE
                     NVDDMRSAITDWSDMREALQYAMGIHADIPPSPERFVATMNPNGSIRMSTLSPSPYRNW
                     QKGGRADPAFLYKVVDGQQSHQAGYANAADRESGIPAAVLDGIKAVAKEKNATLMFRLV
                     NPHSTSLIAEGVATKGLGVHAKSSDWGLQAGYIPVNPNLSKLFGRAPEVIARADNDVNS
                     SLAHGHTAVDLTLSKERLDYLRQAGLVTGMADGVVASNHAGYEQFEFRVKETSDGRYAV
                     QYRRKGGDDFEAVKVIGNAAGIPLTADIDMFAIMPHLSNFRDSARSSVTSGDSVTDYLA
                     RTRRAASEATGGLDRERIDLLWKIARAGARSAVGTEARRQFRYDGDMNIGVITDFELEV
                     RNALNRRAHAVGAQDVVQHGTEQNNPFPEADEKIFVVSATGESQMLTRGQLKEYIGQQR
                     GEGYVFYENRAYGVAGKSLFDDGLGAAPGVPSGRSKFSPDVLETVPASPGLRRPSLGAV
                     ERQDSGYDS"
     misc_feature    912..956
                     /label=linker
                     /note="linker"
     protein_bind    complement(928..952)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    complement(931..951)
                     /label=attB2
                     /note="attB2"
     misc_feature    2190..2297
                     /label=MCS
                     /note="pBluescript multiple cloning site"
     primer_bind     2207..2223
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2257..2273)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        complement(2306..2324)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2334..2350)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             complement(3057..3719)
                     /codon_start=1
                     /gene="rep"
                     /product="REP"
                     /label=rep
                     /note="required for plasmid replication"
                     /protein_id="ABS87318.1"
                     /translation="MATQSREIGIQAKNKPGHWVQTERKAHEAWAGLIARKPTAAMLLH
                     HLVAQMGHQNAVVVSQKTLSKLIGRSLRTVQYAVKDLVAERWISVVKLNGPGTVSAYVV
                     NDRVAWGQPRDQLRLSVFSAAVVVDHDDQDESLLGHGDLRRIPTLYPGEQQLPTGPGEE
                     PPSQPGIPGMEPDLPALTETEEWERRGQQRLPMPDEPCFLDDGEPLEPPTRVTLPRR"
     gene            complement(3057..3719)
                     /gene="rep"
                     /label=rep
     rep_origin      3720..4489
                     /label=pBBR1 oriV
                     /note="replication origin of the broad-host-range plasmid 
                     pBBR1 from Bordetella bronchiseptica; requires the pBBR1 
                     Rep protein for replication"
     misc_feature    complement(4714..5679)
                     /label=mob region
                     /note="mob region"
     CDS             complement(5772..6305)
                     /codon_start=1
                     /gene="GmR"
                     /product="gentamicin-3-acetyltransferase"
                     /label=GmR
                     /protein_id="ABS87319.1"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPRFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     gene            complement(5772..6305)
                     /gene="GmR"
                     /label=GmR