LOCUS       Exported                8766 bp ds-DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector pCPP39, complete sequence.
ACCESSION   HM639781
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8766)
  AUTHORS   Leveau JH, Lindow SE.
  TITLE     Predictive and interpretive simulation of green fluorescent protein 
            expression in reporter bacteria
  JOURNAL   J. Bacteriol. 183 (23), 6752-6762 (2001)
  PUBMED    11698362
REFERENCE   2  (bases 1 to 8766)
  AUTHORS   Remus-Emsermann MN, Leveau JH.
  TITLE     Linking environmental heterogeneity and reproductive success at 
            single-cell resolution
  JOURNAL   ISME J 4 (2), 215-222 (2010)
  PUBMED    19865185
REFERENCE   3  (bases 1 to 8766)
  AUTHORS   Remus-Emsermann MNP., Leveau JHJ.
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUL-2010) Plant Pathology, UC-Davis, One Shields 
            Avenue, Davis, CA 95616-8751, USA
REFERENCE   4  (bases 1 to 8766)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..8766
                     /organism="Cloning vector pCPP39"
                     /mol_type="other DNA"
                     /db_xref="taxon:926020"
     protein_bind    53..74
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     gene            89..465
                     /gene="lacZ-alpha"
                     /label=lacZ-alpha
     regulatory      89..144
                     /regulatory_class="promoter"
                     /gene="lacZ-alpha"
     promoter        89..119
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    127..143
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             169..465
                     /codon_start=1
                     /gene="lacZ-alpha"
                     /product="LacZ-alpha"
                     /label=lacZ-alpha
                     /note="beta-galactosidase"
                     /protein_id="ADQ47508.1"
                     /translation="MITNSSSTAVAAALELVDPPGCRNSISSLSIPSTSRGGPVPMHAS
                     LALAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRSLNGE"
     misc_feature    183..290
                     /label=MCS
                     /note="pBluescript multiple cloning site"
     primer_bind     207..223
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(257..273)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(309..325)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             complement(1452..2102)
                     /codon_start=1
                     /gene="tetR"
                     /product="tetracycline-resistance repressor protein"
                     /label=tetR
                     /protein_id="ADQ47509.1"
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWHF
                     RNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAGTR
                     PGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSDAGERGGTVE
                     QAPLSPLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKGDD"
     gene            complement(1452..2102)
                     /gene="tetR"
                     /label=tetR
     CDS             2208..3407
                     /codon_start=1
                     /gene="tetA"
                     /product="tetracycline-resistance protein"
                     /label=tetA
                     /protein_id="ADQ47510.1"
                     /translation="MKPNIPLIVILSTVALDAVGIGLIMPVLPGLLRDLVHSNDVTAHY
                     GILLALYALVQFACAPVLGALSDRFGRRPILLVSLAGATVDYAIMATAPFLWVLYIGRI
                     VAGITGATGAVAGAYIADITDGDERARHFGFMSACFGFGMVAGPVLGGLMGGFSPHAPF
                     FAAAALNGLNFLTGCFLLPESHKGERRPLRREALNPLASFRWARGMTVVAALMAVFFIM
                     QLVGQVPAALWVIFGEDRFHWDATTIGISLAAFGILHSLAQAMITGPVAARLGERRALM
                     LGMIADGTGYILLAFATRGWMAFPIMVLLASGGIGMPALQAMLSRQVDEERQGQLQGSL
                     AALTSLTSIVGPLLFTAIYAASITTWNGWAWIAGAALYLLCLPALRRGLWSGAGQRADR
                     "
     gene            2208..3407
                     /gene="tetA"
                     /label=tetA
     gene            complement(3462..4610)
                     /gene="lacIq"
                     /label=lacIq
     CDS             complement(3462..4544)
                     /codon_start=1
                     /gene="lacIq"
                     /product="lac-repressor protein"
                     /label=lacIq
                     /protein_id="ADQ47511.1"
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     promoter        complement(4545..4622)
                     /gene="lacI (mutant)"
                     /label=lacIq promoter
                     /note="In the lacIq allele, a single base change in the 
                     promoter boosts expression of the lacI gene about 10-fold."
     regulatory      complement(4579..4610)
                     /regulatory_class="promoter"
                     /gene="lacIq"
     CDS             complement(5924..7072)
                     /codon_start=1
                     /gene="trfA"
                     /product="replication initiator protein A"
                     /label=trfA
                     /protein_id="ADQ47512.1"
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     gene            complement(5924..7072)
                     /gene="trfA"
                     /label=trfA
     CDS             complement(7344..7715)
                     /codon_start=1
                     /gene="traJ"
                     /product="oriV-recognizing protein"
                     /label=traJ
                     /protein_id="ADQ47513.1"
                     /translation="MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSLSAYLLAV
                     GQGYKITGVVDYEHVRELARINGDLGRLGGLLKLWLTDDPRTARFGDATILALLAKIEE
                     KQDELGKVMMGVVRPRAEP"
     gene            complement(7344..7715)
                     /gene="traJ"
                     /label=traJ
     oriT            7748..7857
                     /note="incP origin of transfer"
     rep_origin      8010..8641
                     /label=oriV
                     /note="incP origin of replication"
     rep_origin      complement(8012..8641)
                     /direction=LEFT
                     /label=oriV
                     /note="oriV"