LOCUS       Exported                6997 bp ds-DNA     circular SYN 17-DEC-2018
DEFINITION  Expression vector pCPC77, complete sequence.
ACCESSION   MG883679
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6997)
  AUTHORS   Chang P, Wang W, Chen J.
  TITLE     An efficient vector system for economical and convenient 
            epitope-tagging and promoters switching in Candida albicans
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6997)
  AUTHORS   Chang P, Wang W, Chen J.
  TITLE     Direct Submission
  JOURNAL   Submitted (31-JAN-2018) Research Center of Bioenergy & 
            Bioremediation, Southwest University, 2 Tiansheng Road, Beibei 
            District, Chongqiong, Chongqing 400715, China
REFERENCE   3  (bases 1 to 6997)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..6997
                     /organism="Expression vector pCPC77"
                     /mol_type="other DNA"
                     /db_xref="taxon:2136778"
     regulatory      78..856
                     /regulatory_class="promoter"
                     /note="tet_OP4 promoter; tet promoter"
     protein_bind    201..471
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     repeat_region   905..991
                     /note="tandem HA tag repeats; 3X HA"
     CDS             905..931
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             935..961
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             965..991
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     rep_origin      complement(1630..2218)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2389..3249)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(3250..3354)
                     /gene="bla"
                     /label=AmpR promoter
     regulatory      3425..4159
                     /regulatory_class="promoter"
                     /note="promoter region of ADH1 gene of Candida albicans; 
                     CaADH1p"
     CDS             4160..5143
                     /codon_start=1
                     /product="TetR/Gal4AD fusion protein"
                     /label=TetR/Gal4AD fusion protein
                     /note="Candida albicans codon-optimized"
                     /protein_id="AVR52652.1"
                     /translation="MSRLDKSKVINSALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHSCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLKQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGSTLFGGANFNQSGNI
                     ADSSLSFTFTNSSNGPNLITTQTNSQALSQPIASSNVHDNFMNNEITASKIDDGNNSKP
                     LSPGWTDQTAYNAFGITTGMFNTTTMDDVYNYLFDDEDTPPNPKKE"
     CDS             4799..5143
                     /codon_start=1
                     /gene="Saccharomyces cerevisiae GAL4 (truncated)"
                     /product="activation domain of the GAL4 transcriptional 
                     activator"
                     /label=GAL4 activation domain
                     /translation="ANFNQSGNIADSSLSFTFTNSSNGPNLITTQTNSQALSQPIASSN
                     VHDNFMNNEITASKIDDGNNSKPLSPGWTDQTAYNAFGITTGMFNTTTMDDVYNYLFDD
                     EDTPPNPKKE"
     regulatory      5158..5464
                     /regulatory_class="terminator"
                     /note="terminator region of ACT1 gene of Candida albicans; 
                     ACT1 terminator"
     CDS             5926..6822
                     /codon_start=1
                     /product="His1"
                     /label=His1
                     /protein_id="AVR52653.1"
                     /translation="MDLVNHLPDRLLFAVPKKGRLYEKCCNLLSGADIQFRRSNRLDIA
                     LSTNLPIALIFLPAADIPVFVGEGNCDLGITGLDQIKEAEQFDNIEDLLDLKFGSCKLQ
                     IQVPADGEYEKPEQLVGKKIVSSFTKLSTDYFKQLSDKPTNIRYVGGSVEASCALGVAD
                     AIVDLVESGETMKAAGLKAIETILETSAHLISSKKSKFPEMVNIIVQRLQGVLAAQEYV
                     LCNYNAPKSIQAKCLTITPGRRAATVSTLDKHSDDEEDWVAISSMVNRKEIGNVMDELK
                     KAGATDILVLEISNCRV"