LOCUS       Exported                6432 bp ds-DNA     circular SYN 17-DEC-2018
DEFINITION  Expression vector pCPC66, complete sequence.
ACCESSION   MG883678
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6432)
  AUTHORS   Chang P, Wang W, Chen J.
  TITLE     An efficient vector system for economical and convenient 
            epitope-tagging and promoters switching in Candida albicans
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6432)
  AUTHORS   Chang P, Wang W, Chen J.
  TITLE     Direct Submission
  JOURNAL   Submitted (31-JAN-2018) Research Center of Bioenergy & 
            Bioremediation, Southwest University, 2 Tiansheng Road, Beibei 
            District, Chongqiong, Chongqing 400715, China
REFERENCE   3  (bases 1 to 6432)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..6432
                     /organism="Expression vector pCPC66"
                     /mol_type="other DNA"
                     /db_xref="taxon:2136777"
     CDS             complement(552..1412)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(1413..1517)
                     /gene="bla"
                     /label=AmpR promoter
     misc_feature    1621..2328
                     /note="similar to yeast-enhanced green fluorescent protein;
                     codons optimized for translation in S. cerevisiae and C. 
                     albicans; yeGFP"
     CDS             1621..2328
                     /codon_start=1
                     /product="yeast-enhanced green fluorescent protein"
                     /label=yeGFP
                     /note="codons optimized for translation in S. cerevisiae 
                     and C. albicans"
                     /translation="SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKF
                     ICTTGKLPVPWPTLVTTFGYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGN
                     YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVN
                     FKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEF
                     VTAAGITHGMDELY"
     regulatory      2356..2662
                     /regulatory_class="terminator"
                     /note="terminator region of ACT1 gene of Candida albicans; 
                     ACT1 terminator"
     protein_bind    2699..2732
                     /label=Cre recombinase binding site
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT); loxP"
     CDS             3814..5208
                     /codon_start=1
                     /product="Arg4"
                     /label=Arg4
                     /protein_id="AVR52651.1"
                     /translation="MSQQPSENKLWGGRFTGATDPLMDLYNASLPYDKVMYDADLTGTK
                     VYTQGLNKLGLITTEELNLIHQGLEQIRQEWHDNKFIIKAGDEDIHTANERRLGEIIGK
                     NISGKVHTGRSRNDQVATDMRIFVRESLLNLSKILHQFITAILERAHKEIDVLMPGYTH
                     LQKAQPIRWAHWLSSYATYFTEDYKRLQEIITRVNQSPLGSGALAGHPYGIDREFLAKG
                     LGFDGVIGNSLTAVSDRDFVVESLFWSTLFMNHISRFSEDLIIYSSGEFGFIKLADAYS
                     TGSSLMPQKKNPDSLELLRGKSGRVFGQLSGFLMSIKSIPSTYNKDMQEDKEPLFDALT
                     TVEHSILIATGVISTLLIDKQNMEKALTMDMLATDLADYLVRKGVPFRETHHISGECVR
                     KAEEEKLSGIDQLSFEQFQQIDSRFEKDVMETFDFEASVERRDAIGGTAKSAVLKQLEN
                     LKSILN"
     protein_bind    6026..6059
                     /label=Cre recombinase binding site
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT); loxP"
     rep_origin      complement(6225..381)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"