LOCUS       Exported                4284 bp ds-DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector pCPC49, complete sequence.
ACCESSION   MG874800
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4284)
  AUTHORS   Chang P, Wang W, Chen J.
  TITLE     Fast and convenient doxycycline-induced marker recycling in Candida 
            albicans via Cre-loxP system
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4284)
  AUTHORS   Chang P, Wang W, Chen J.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2018) Research Center of Bioenergy & 
            Bioremediation, Southwest University, 2 Tiansheng Road, Chongqiong, 
            Chongqing 400075, China
REFERENCE   3  (bases 1 to 4284)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..4284
                     /organism="Cloning vector pCPC49"
                     /mol_type="other DNA"
                     /db_xref="taxon:2136742"
     rep_origin      complement(164..3860)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(335..1195)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(1196..1300)
                     /gene="bla"
                     /label=AmpR promoter
     protein_bind    1339..1372
                     /standard_name="loxP"
                     /label=loxP binding site
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)"
     CDS             2448..3344
                     /codon_start=1
                     /gene="HIS1"
                     /product="His1"
                     /label=HIS1
                     /protein_id="AVR52676.1"
                     /translation="MDLVNHLPDRLLFAVPKKGRLYEKCCSLLSGADIQFRRSNRLDIA
                     LSTNLPIALIFLPAADIPVFVGEGNCDLGITGLDQIKEADQFDNIEDLLDLKFGSCKLQ
                     IQVPANGEYEKPEQLVGKKIVSSFTKLSTDYFKKLSDKPTNIRYVGGSVEASCALGVAD
                     AIVDLVESGETMKAAGLKAIETILETSAHLISSKKTKFPEMVNIIVQRLQGVLAAQEYV
                     LCNYNAPKSIQSKCLTITPGRRAATVSTLDKHSDDEDDWVAISSMVNRKEIGNVMDELK
                     KAGATDILVLEISNCRV"
     gene            2448..3344
                     /gene="HIS1"
                     /label=HIS1
     protein_bind    3721..3754
                     /label=Cre recombinase binding site
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)"
     rep_origin      complement(3860..164)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"