LOCUS       Exported                4203 bp ds-DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector pCPC48, complete sequence.
ACCESSION   MG874799
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4203)
  AUTHORS   Chang P, Wang W, Chen J.
  TITLE     Fast and convenient doxycycline-induced marker recycling in Candida 
            albicans via Cre-loxP system
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4203)
  AUTHORS   Chang P, Wang W, Chen J.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2018) Research Center of Bioenergy & 
            Bioremediation, Southwest University, 2 Tiansheng Road, Chongqiong, 
            Chongqing 400075, China
REFERENCE   3  (bases 1 to 4203)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..4203
                     /organism="Cloning vector pCPC48"
                     /mol_type="other DNA"
                     /db_xref="taxon:2136741"
     promoter        complement(476..580)
                     /gene="bla"
                     /label=AmpR promoter
     protein_bind    619..652
                     /label=Cre recombinase binding site
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence"
     CDS             1549..2670
                     /codon_start=1
                     /gene="LEU2"
                     /product="Leu2"
                     /label=LEU2
                     /protein_id="AVR52675.1"
                     /translation="MSVKTKTITILPGDHVGTEIVNEAIKVLEAIEAATPYQKIHFDFK
                     HHLIGGAAIDATGVPLPDDALESAKKSDAVLLGAVGGPKWGTGAVRPEQGLLKIRKELN
                     LYANIRPCNFASDSLLELSPLRPEVVKGTNLIIVRELVGGIYFGDREEQEESEDKQTAW
                     DTEKYTVDEVTRITRMAAFMALQHNPPLPIWSLDKANVLASSRLWRRTVDKVISEEFPT
                     LSVQHQLIDSAAMILIQNPTKLNGIIITSNMFGDIISDEASVIPGSLGLLPSASLASLP
                     DTNTAFGLYEPCHGSAPDLPANKVNPIATILSAASMLRLSLDCVKEAEALEEAVKQVLD
                     KGIRTADLRGTSSATEVGDAIVEAVTKILKEKA"
     gene            1549..2670
                     /gene="LEU2"
                     /label=LEU2
     protein_bind    2920..2953
                     /label=Cre recombinase binding site
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence"
     rep_origin      complement(3059..3647)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3818..475)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"