LOCUS       Exported                7255 bp ds-DNA     circular SYN 17-DEC-2018
DEFINITION  Expression vector pCPC28, complete sequence.
ACCESSION   MG883662
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7255)
  AUTHORS   Chang P, Wang W, Chen J.
  TITLE     An efficient vector system for economical and convenient 
            epitope-tagging and promoters switching in Candida albicans
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7255)
  AUTHORS   Chang P, Wang W, Chen J.
  TITLE     Direct Submission
  JOURNAL   Submitted (31-JAN-2018) Research Center of Bioenergy & 
            Bioremediation, Southwest University, 2 Tiansheng Road, Beibei 
            District, Chongqiong, Chongqing 400715, China
REFERENCE   3  (bases 1 to 7255)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..7255
                     /organism="Expression vector pCPC28"
                     /mol_type="other DNA"
                     /db_xref="taxon:2136761"
     misc_feature    complement(241..6971)
                     /note="3 region of EAF7 genes of Candida albicans; 3 of 
                     EAF7"
     rep_origin      complement(352..940)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(1111..1971)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(1972..2076)
                     /gene="bla"
                     /label=AmpR promoter
     misc_feature    2178..2622
                     /note="5 region of EAF7 genes of Candida albicans; 5 of 
                     EAF7"
     regulatory      2638..4110
                     /regulatory_class="promoter"
                     /note="promoter region of ADH1 gene of Candida albicans; 
                     CaADH1p"
     regulatory      4124..4430
                     /regulatory_class="terminator"
                     /note="terminator region of ACT1 gene of Candida albicans; 
                     ACT1 terminator"
     protein_bind    4467..4500
                     /label=Cre recombinase binding site
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT); loxP"
     CDS             5576..6472
                     /codon_start=1
                     /product="His1"
                     /label=His1
                     /protein_id="AVR52629.1"
                     /translation="MDLVNHLPDRLLFAVPKKGRLYEKCCSLLSGADIQFRRSNRLDIA
                     LSTNLPIALIFLPAADIPVFVGEGNCDLGITGLDQIKEADQFDNIEDLLDLKFGSCKLQ
                     IQVPANGEYEKPEQLVGKKIVSSFTKLSTDYFKKLSDKPTNIRYVGGSVEASCALGVAD
                     AIVDLVESGETMKAAGLKAIETILETSAHLISSKKTKFPEMVNIIVQRLQGVLAAQEYV
                     LCNYNAPKSIQSKCLTITPGRRAATVSTLDKHSDDEDDWVAISSMVNRKEIGNVMDELK
                     KAGATDILVLEISNCRV"
     protein_bind    6849..6882
                     /label=Cre recombinase binding site
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT); loxP"