LOCUS       Exported                7174 bp ds-DNA     circular SYN 17-DEC-2018
DEFINITION  Expression vector pCPC27, complete sequence.
ACCESSION   MG883661
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7174)
  AUTHORS   Chang P, Wang W, Chen J.
  TITLE     An efficient vector system for economical and convenient 
            epitope-tagging and promoters switching in Candida albicans
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7174)
  AUTHORS   Chang P, Wang W, Chen J.
  TITLE     Direct Submission
  JOURNAL   Submitted (31-JAN-2018) Research Center of Bioenergy & 
            Bioremediation, Southwest University, 2 Tiansheng Road, Beibei 
            District, Chongqiong, Chongqing 400715, China
REFERENCE   3  (bases 1 to 7174)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..7174
                     /organism="Expression vector pCPC27"
                     /mol_type="other DNA"
                     /db_xref="taxon:2136760"
     rep_origin      complement(52..640)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(811..1671)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(1672..1776)
                     /gene="bla"
                     /label=AmpR promoter
     misc_feature    1878..2322
                     /note="5 region of EAF7 genes of Candida albicans; 5 of 
                     EAF7"
     regulatory      2338..3810
                     /regulatory_class="promoter"
                     /note="promoter region of ADH1 gene of Candida albicans; 
                     CaADH1p"
     regulatory      3824..4130
                     /regulatory_class="terminator"
                     /note="terminator region of ACT1 gene of Candida albicans; 
                     ACT1 terminator"
     protein_bind    4167..4200
                     /label=Cre recombinase binding site
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT); loxP"
     CDS             5097..6218
                     /codon_start=1
                     /product="Leu2"
                     /label=Leu2
                     /protein_id="AVR52628.1"
                     /translation="MSVKTKTITILPGDHVGTEIVNEAIKVLEAIEAATPYQKIHFDFK
                     HHLIGGAAIDATGVPLPDDALESAKKSDAVLLGAVGGPKWGTGAVRPEQGLLKIRKELN
                     LYANIRPCNFASDSLLELSPLRPEVVKGTNLIIVRELVGGIYFGDREEQEESEDKQTAW
                     DTEKYTVDEVTRITRMAAFMALQHNPPLPIWSLDKANVLASSRLWRRTVDKVISEEFPT
                     LSVQHQLIDSAAMILIQNPTKLNGIIITSNMFGDIISDEASVIPGSLGLLPSASLASLP
                     DTNTAFGLYEPCHGSAPDLPANKVNPIATILSAASMLRLSLDCVKEAEALEEAVKQVLD
                     KGIRTADLRGTSSATEVGDAIVEAVTKILKEKA"
     protein_bind    6468..6501
                     /label=Cre recombinase binding site
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT); loxP"
     misc_feature    6590..7115
                     /note="3 region of EAF7 genes of Candida albicans; 3 of 
                     EAF7"