LOCUS       Exported                7655 bp ds-DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector pCotZ-CL-thr, complete sequence.
ACCESSION   KF933412
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7655)
  AUTHORS   Iwanicki A, Hinc K, Stasilojc M, Piatek I, Grela A, Lega T, 
            Obuchowski M.
  TITLE     Vector system for spore sufrace display
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7655)
  AUTHORS   Iwanicki A, Hinc K, Stasilojc M, Piatek I, Grela A, Lega T, 
            Obuchowski M.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2013) Laboratory of Molecular Bacteriology, 
            Medical University of Gdansk, Debinki 1, Gdansk 80-211, Poland
REFERENCE   3  (bases 1 to 7655)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Assembly Method       :: Vector NTI Advanced v. 10
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7655
                     /organism="Cloning vector pCotZ-CL-thr"
                     /mol_type="other DNA"
                     /db_xref="taxon:1449705"
     CDS             247..879
                     /codon_start=1
                     /gene="thrC"
                     /product="ThrC"
                     /label=thrC
                     /note="N terminal part of thrC"
                     /protein_id="AHG54660.1"
                     /translation="MWKGLIHQYKEFLPVTDQTPALTLHEGNTPLIHLPKLSEQLGIEL
                     HVKTEGVNPTGSFKDRGMVMAVAKAKEEGNDTIMCASTGNTSAAAAAYAARANMKCIVI
                     IPNGKIAFGKLAQAVMYGAEIIAIDGNFDDALKIVRSICEKSPIALVNSVNPYRIEGQK
                     TAAFEIVHNGFPYAKYGQTWPARLLLFLTPDQLVMVATGAQLELRRY"
     gene            247..879
                     /gene="thrC"
                     /label=thrC
     gene            complement(1124..2316)
                     /gene="cotZ"
                     /label=cotZ
     misc_feature    complement(1124..1149)
                     /gene="cotZ"
                     /label=multiple cloning site
                     /note="multiple cloning site"
     CDS             complement(1135..1626)
                     /codon_start=1
                     /gene="cotZ"
                     /product="CotZ"
                     /label=cotZ
                     /note="spore coat protein"
                     /protein_id="AHG54656.1"
                     /translation="MSQKTSSCVREAVENIEDLQNAVEEDCPTGCHSKLLSVSHSLGDT
                     VPFAIFTSKSTPLVAFGNVGELDNGPCFNTVFFRVERVHGSCATLSLLIAFDEHKHILD
                     FTDKDTVCEVFRLEKTNYCIEVDLDCFCAINCLNPRLINRTHHHGGGEAAAKGGGGSGR
                     "
     regulatory      complement(1633..1638)
                     /regulatory_class="ribosome_binding_site"
                     /gene="cotZ"
     CDS             complement(1754..2293)
                     /codon_start=1
                     /gene="cotY"
                     /product="CotY"
                     /label=cotY
                     /note="spore coat protein"
                     /protein_id="AHG54655.1"
                     /translation="MLLRTKVKYPIKKELKSMSCGKTHGRHENCVCDAVEKILAEQEAV
                     EEQCPTGCYTNLLNPTIAGKDTIPFLVFDKKGGLFSTFGNVGGFVDDMQCFESIFFRVE
                     KLCDCCATLSILRPVDVKGDTLSVCHPCDPDFFGLEKTDFCIEVDLGCFCAIQCLSPEL
                     VDRTSPHKDKKHHHNG"
     gene            complement(1754..2293)
                     /gene="cotY"
                     /label=cotY
     regulatory      complement(2288..2316)
                     /regulatory_class="promoter"
                     /gene="cotZ"
     CDS             2536..3288
                     /codon_start=1
                     /gene="trpC"
                     /product="TrpC"
                     /label=trpC
                     /protein_id="AHG54661.1"
                     /translation="MLEKIIKQKKEEVKTLVLPVEQPFEKRSFKEAPASPNRFIGLIAE
                     VKKASPSKGLIKEDFVPVQIAKDYEAAKADAISVLTDTPFFQGENSYLSDVKRAVSIPV
                     LRKDFIIDSLQVEESRRIGADAILLIGEVLDPLHLHELYLEAGEKGMDVLVEVHDASTL
                     EQILKVFTPDILGVNNRNLKTFETSVKQTEQIASLVPKESLLVSESGIGSLEHLTFVNE
                     HGARAVLIGESLMRQTSQRKAIHALFRE"
     gene            2536..3288
                     /gene="trpC"
                     /label=trpC
     CDS             3718..4146
                     /codon_start=1
                     /gene="thrC"
                     /product="ThrC"
                     /label=thrC
                     /note="C-terminal part of thrC"
                     /protein_id="AHG54659.1"
                     /translation="MRGFEAEGAAAIVRNEVIENPETIATAIRIGNPASWDKAVKAAEE
                     SNGKIDEVTDDEILHAYQLIARVEGVFAEPGSCASIAGVLKQVKSGEIPKGSKVVAVLT
                     GNGLKDPNTAVDISEIKPVTLPTDEDSILEYVKGAARV"
     gene            3718..4146
                     /gene="thrC"
                     /label=thrC
     CDS             4143..4553
                     /codon_start=1
                     /gene="thrB"
                     /product="ThrB"
                     /label=thrB
                     /note="N terminal part of thrB"
                     /protein_id="AHG54658.1"
                     /translation="MNEADMLFSVTVPGSTANLGPGFDSVGMALSRYLKLTVFESDKWS
                     FEAETETVAGINSSSFNFLTSSCIGTISRSSNPSSFTSIRFNQHSKNLTGHRQFFEQCA
                     NYQLFLQFIQGTGEPACNPSCSIMRNDPHPVS"
     gene            4143..4553
                     /gene="thrB"
                     /label=thrB
     CDS             4797..5579
                     /codon_start=1
                     /gene="spc"
                     /product="Spc"
                     /label=spc
                     /note="resistance for spectomycine"
                     /protein_id="AHG54657.1"
                     /translation="MSNLINGKIPNQAIQTLKIVKDLFGSSIVGVYLFGSAVNGGLRIN
                     SDVDVLVVVNHSLPQLTRKKLTERLMTISGKIGNTDSVRPLEVTVINRSEVVPWQYPPK
                     REFIYGEWLRGEFENGQIQEPSYDPDLAIVLAQARKNSISLFGPDSSSILVSVPLTDIR
                     RAIKDSLPELIEGIKGDERNVILTLARMWQTVTTGEITSKDVAAEWAIPLLPKEHVTLL
                     DIARKGYRGECDDKWEGLYSKVKALVKYMKNSIETSLN"
     gene            4797..5579
                     /gene="spc"
                     /label=spc
     rep_origin      complement(5818..6490)
                     /direction=LEFT
                     /label=ColE1
                     /note="ColE1"
     rep_origin      complement(5826..6414)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(6585..7445)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=bla
                     /protein_id="AHG54654.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     gene            complement(6585..7445)
                     /gene="bla"
                     /label=bla
     promoter        complement(7446..7550)
                     /gene="bla"
                     /label=AmpR promoter