LOCUS       Exported                6340 bp ds-DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector pCotG-NL, complete sequence.
ACCESSION   KF933406
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6340)
  AUTHORS   Iwanicki A, Hinc K, Stasilojc M, Piatek I, Grela A, Lega T, 
            Obuchowski M.
  TITLE     Vector system for spore sufrace display
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6340)
  AUTHORS   Iwanicki A, Hinc K, Stasilojc M, Piatek I, Grela A, Lega T, 
            Obuchowski M.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-OCT-2013) Laboratory of Molecular Bacteriology, 
            Medical University of Gdansk, Debinki 1, Gdansk 80-211, Poland
REFERENCE   3  (bases 1 to 6340)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Assembly Method       :: Vector NTI Advanced v. 10
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6340
                     /organism="Cloning vector pCotG-NL"
                     /mol_type="other DNA"
                     /db_xref="taxon:1449701"
     CDS             372..1691
                     /codon_start=1
                     /gene="lysA"
                     /product="LysA"
                     /label=lysA
                     /note="diaminopimelate decarboxylase"
                     /protein_id="AHG54623.1"
                     /translation="MFLHGTSRQNQHGHLEIGGVDALYLAEKYGTPLYVYDVALIRERA
                     KSFKQAFISAGLKAQVAYASKAFSSVAMIQLAEEEGLSLDVVSGGELYTAVAAGFPAER
                     IHFHGNNKSREELRMALEHRIGCIVVDNFYEIALLEDLCKETGHSIDVLLRITPGVEAH
                     THDYITTGQEDSKFGFDLHNGQTERAIEQVLQSEHIQLLGVHCHIGSQIFDTAGFVLAA
                     EKIFKKLDEWRDSYSFVSKVLNLGGGFGIRYTEDDEPLHATEYVEKIIEAVKENASRYG
                     FDIPEIWIEPGRSLVGDAGTTLYTVGSQKEVPGVRQYVAVDGGMNDNIRPALYQAKYEA
                     AAANRIGEAHDKTVSIAGKCCESGDMLIWDIDLPEVKEGDLLAVFCTGAYGYSMANNYN
                     RIPRPAVVFVENGEAHLVVKRETYEDIVKLDLPFKTGVKQ"
     gene            372..1691
                     /gene="lysA"
                     /label=lysA
     misc_recomb     complement(1806..2192)
                     /label=pyrD
                     /note="pyrD"
     promoter        2714..2818
                     /gene="bla"
                     /label=AmpR promoter
     CDS             2819..3679
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=bla
                     /protein_id="AHG54621.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     gene            2819..3679
                     /gene="bla"
                     /label=bla
     rep_origin      3774..4456
                     /label=ColE1
                     /note="ColE1"
     rep_origin      3850..4438
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_recomb     complement(4666..5133)
                     /label=pyrD
                     /note="pyrD"
     gene            5633..6335
                     /gene="cotG"
                     /label=cotG
     regulatory      5633..5675
                     /regulatory_class="promoter"
                     /gene="cotG"
     regulatory      5708..5713
                     /regulatory_class="ribosome_binding_site"
                     /gene="cotG"
     CDS             5721..6335
                     /codon_start=1
                     /gene="cotG"
                     /product="CotG"
                     /label=cotG
                     /note="spore coat protein"
                     /protein_id="AHG54622.1"
                     /translation="MGILELGGGGSHYSHSDIEEAVKSAKKEGLKDYLYQEPHGKKRSH
                     KKSHRTHKKSRSHKKSYCSHKKSRSHKKSFCSHKKSRSHKKSYCSHKKSRSHKKSYRSH
                     KKSRSYKKSYRSYKKSRSYKKSCRSYKKSRSYKKSYCSHKKKSRSYKKSCRTHKKSYRS
                     HKKYYKKPHHHCDDYKRHDDYDSKKEYWKDGNCWVVKKKYK"
     misc_feature    5725..5738
                     /gene="cotG"
                     /label=multiple cloning site
                     /note="multiple cloning site"