LOCUS       Exported                5465 bp ds-DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector pCotC-N, complete sequence.
ACCESSION   KF933401
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5465)
  AUTHORS   Iwanicki A, Hinc K, Stasilojc M, Piatek I, Grela A, Lega T, 
            Obuchowski M.
  TITLE     Vector system for spore sufrace display
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5465)
  AUTHORS   Iwanicki A, Hinc K, Stasilojc M, Piatek I, Grela A, Lega T, 
            Obuchowski M.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-OCT-2013) Laboratory of Molecular Bacteriology, 
            Medical University of Gdansk, Debinki 1, Gdansk 80-211, Poland
REFERENCE   3  (bases 1 to 5465)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Assembly Method       :: Vectro NTI Advanced v. 10
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5465
                     /organism="Cloning vector pCotC-N"
                     /mol_type="other DNA"
                     /db_xref="taxon:1449696"
     primer_bind     379..395
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_recomb     complement(401..873)
                     /label=C-terminal part of lacA
                     /note="C-terminal part of lacA"
     gene            986..1268
                     /gene="cotC"
                     /label=cotC
     regulatory      986..1032
                     /regulatory_class="promoter"
                     /gene="cotC"
     regulatory      1040..1045
                     /regulatory_class="ribosome_binding_site"
                     /gene="cotC"
     CDS             1053..1268
                     /codon_start=1
                     /gene="cotC"
                     /product="CotC"
                     /label=cotC
                     /note="spore coat protein"
                     /protein_id="AHG54607.1"
                     /translation="MGTRGSGYYKKYKEEYYTVKKTYYKKYYEYDKKDYDCDYDKKYDD
                     YDKKYYDHDKKDYDYVVEYKKHKKHY"
     misc_feature    1056..1070
                     /gene="cotC"
                     /label=multiple cloning site
                     /note="multiple cloning site"
     gene            1333..2828
                     /gene="hisD"
                     /label=hisD
     regulatory      1333..1518
                     /regulatory_class="promoter"
                     /gene="hisD"
                     /note="promoter region"
     CDS             1545..2828
                     /codon_start=1
                     /gene="hisD"
                     /product="HisD"
                     /label=hisD
                     /note="histidinol dehydrogenase"
                     /protein_id="AHG54608.1"
                     /translation="MKIKTISGAERLSLKRSIDAGTEEQRKTVRSIIEDVKANGDQAVR
                     SYTAKFDCIEIDSPLVTKEEFEEAYTSLDSRLLQVIRQAIENIREYHERQLQSSWFYHR
                     KDGTMLGQKVTALDSAGVYVPGGTAAYPSSVLMNVIPALVAGVERIVLVTPPGKDGLLS
                     PGVLVAAAELGIKDIYKMGGAQAIAALAYGTETIEPVDKITGPGNIYVALAKREVFGDV
                     DIDMIAGPSEIVVLADETAIPSEIAADLLSQAEHDKLSSCVFVTDSMALAETVSAEVNK
                     QLETLPRREIAEASVRDYGCIYVAETMVEAIETVNTLAPEHLEIITQSPEALLGSIKHA
                     GAIFLGRYSPEPVGDYFAGPNHVLPTNGTARFSSPLNVTDFQKKSSIISYSQSAFEEHA
                     ESIAAFARLEGLEAHARSIEARERRISK"
     misc_recomb     complement(2847..3225)
                     /label=N terminal part of lacA
                     /note="N terminal part of lacA"
     primer_bind     complement(3244..3260)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    3268..3284
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(3292..3322)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3337..3358
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(3638..4310)
                     /direction=LEFT
                     /label=ColE1
                     /note="ColE1"
     rep_origin      complement(3646..4234)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(4405..5265)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=bla
                     /protein_id="AHG54606.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     gene            complement(4405..5265)
                     /gene="bla"
                     /label=bla
     promoter        complement(5266..5370)
                     /gene="bla"
                     /label=AmpR promoter