LOCUS       Exported                8257 bp ds-DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector pCotB-CL, complete sequence.
ACCESSION   KF933396
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8257)
  AUTHORS   Iwanicki A, Hinc K, Stasilojc M, Piatek I, Grela A, Lega T, 
            Obuchowski M.
  TITLE     Vector system for spore sufrace display
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8257)
  AUTHORS   Iwanicki A, Hinc K, Stasilojc M, Piatek I, Grela A, Lega T, 
            Obuchowski M.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-OCT-2013) Laboratory of Molecular Bacteriology, 
            Medical University of Gdansk, Debinki 1, Gdansk 80-211, Poland
REFERENCE   3  (bases 1 to 8257)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Assembly Method       :: Vectro NTI Advanced v. 10
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..8257
                     /organism="Cloning vector pCotB-CL"
                     /mol_type="other DNA"
                     /db_xref="taxon:1449691"
     promoter        106..210
                     /gene="bla"
                     /label=AmpR promoter
     CDS             211..1071
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=bla
                     /protein_id="AHG54590.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     gene            211..1071
                     /gene="bla"
                     /label=bla
     rep_origin      1166..1838
                     /label=ColE1
                     /note="ColE1"
     rep_origin      1242..1830
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    2016..2156
                     /label=bom
                     /note="basis of mobility region from pBR322"
     CDS             complement(2258..2449)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     CDS             complement(3705..4724)
                     /codon_start=1
                     /gene="amyE"
                     /product="AmyE"
                     /label=amyE
                     /note="C-terminal part of amyE"
                     /protein_id="AHG54588.1"
                     /translation="MSDDDIRLGWAVIASRSGSTPLFFSRPEGGGNGVRFPGKSQIGDR
                     GSALFEDQAITAVNRFHNVMAGQPEELSNPNGNNQIFMNQRGSHGVVLANAGSSSVSIN
                     TATKLPDGRYDNKAGAGSFQVNDGKLTGTINARSVAVLYPDDIAKAPHVFLENYKTGVT
                     HSFNDQLTITLRADANTTKAVYQINNGPDDRRLRMEINSQSEKEIQFGKTYTIMLKGTN
                     SDGVTRTEKYSFVKRDPASAKTIGYQNPNHWSQVNAYIYKHDGSRVIELTGSWPGKPMT
                     KNADGIYTLTLPADTDTTNAKVIFNNGSAQVPGQNQPGFDYVLNGLYNDSGLSGSLPH"
     gene            complement(3705..4724)
                     /gene="amyE"
                     /label=amyE
     misc_feature    4825..4959
                     /gene="cotH"
                     /note="cotH; C-terminal part of cotH"
     gene            4825..4959
                     /gene="cotH"
                     /label=cotH
     gene            5011..5973
                     /gene="cotB"
                     /label=cotB
     regulatory      5011..5050
                     /regulatory_class="promoter"
                     /gene="cotB"
     regulatory      5074..5081
                     /regulatory_class="ribosome_binding_site"
                     /gene="cotB"
     CDS             5087..5965
                     /codon_start=1
                     /gene="cotB"
                     /product="CotB"
                     /label=cotB
                     /note="spore coat protein"
                     /protein_id="AHG54591.1"
                     /translation="MSKRRMKTRSNNEISYYNFLHSMKDKIVTVYRGGPESKKGKLTAV
                     KSDYIALQAEKKIIYYQLEHVKSITEDTNNSTTTIETEEMLDADDFHSLIGHLINQSVQ
                     FNQGGPESKKGRLVWLGDDYAALNTNEDGVVYFNIHHIKSISKHEPDLKIEEQTPVGVL
                     EADDLSEVFKSLTHKWVSINRGGPEAIEGILVDNADGHYTIVKNQEVLRIYPFHIKSIS
                     LGPKGSYKKEDQKNEQNQEDNNDKDSNSFISSKSYSSSKSSKRSLKSSDDQSSGSWNSG
                     GGSRIPWARGR"
     misc_feature    5939..5973
                     /gene="cotB"
                     /label=multiple cloning site
                     /note="multiple cloning site"
     gene            6065..7250
                     /gene="thrC"
                     /label=thrC
     regulatory      6065..6093
                     /regulatory_class="promoter"
                     /gene="thrC"
     CDS             6191..7249
                     /codon_start=1
                     /gene="thrC"
                     /product="ThrC"
                     /label=thrC
                     /protein_id="AHG54592.1"
                     /translation="MWKGLIHQYKEFLPVTDQTPALTLHEGNTPLIHLPKLSEQLGIEL
                     HVKTEGVNPTGSFKDRGMVMAVAKAKEEGNDTIMCASTGNTSAAAAAYAARANMKCIVI
                     IPNGKIAFGKLAQAVMYGAEIIAIDGNFDDALKIVRSICEKSPIALVNSVNPYRIEGQK
                     TAAFEVCEQLGEAPDVLAIPVGNAGNITAYWKGFKEYHEKNGTGLPKMRGFEAEGAAAI
                     VRNEVIENPETIATAIRIGNPASWDKAVKAAEESNGKIDEVTDDEILHAYQLIARVEGV
                     FAEPGSCASIAGVLKQVKSGEIPKGSKVVAVLTGNGLKDPNTAVDISEIKPVTLPTDED
                     SILEYVKGAARV"
     CDS             complement(7610..8143)
                     /codon_start=1
                     /gene="amyE"
                     /product="AmyE"
                     /label=amyE
                     /note="N terminal part of amyE"
                     /protein_id="AHG54589.1"
                     /translation="MFAKRFKTSLLPLFAGFLLLFHLVLAGPAAASAETANKSNELTAP
                     SIKSGTILHAWNWSFNTLKHNMKDIHDAGYTAIQTSPINQVKEGNQGDKSMSNWYWLYQ
                     PTSYQIGNRYLGTEQEFKEMCAAAEEYGIKVIVDAVINHTTSDYAAISNEVKSIPNWTH
                     GNTQIKNWSDRNST"
     gene            complement(7610..8143)
                     /gene="amyE"
                     /label=amyE