LOCUS       Exported                3999 bp ds-DNA     circular SYN 17-DEC-2018
DEFINITION  Integrative shuttle vector pCONINT, complete sequence.
ACCESSION   DQ191756
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3999)
  AUTHORS   Dennehy M, Bourn W, Steele D, Williamson AL.
  TITLE     Evaluation of recombinant BCG expressing rotavirus VP6 as an 
            anti-rotavirus vaccine
  JOURNAL   Vaccine 25 (18), 3646-3657 (2007)
  PUBMED    17339069
REFERENCE   2  (bases 1 to 3999)
  AUTHORS   Bourn W.
  TITLE     Direct Submission
  JOURNAL   Submitted (27-AUG-2005) Medical Virology, University of Cape Town, 
            Anzio Road, Observatory, Cape Town 7925, South Africa
REFERENCE   3  (bases 1 to 3999)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..3999
                     /organism="Integrative shuttle vector pCONINT"
                     /mol_type="other DNA"
                     /label=Mycobacterium-E.coli integrative shuttle vector
                     /note="Mycobacterium-E.coli integrative shuttle vector"
                     /db_xref="taxon:345409"
     CDS             120..935
                     /codon_start=1
                     /product="aminoglycoside-3-phosphotransferase"
                     /function="kanamycin resistance"
                     /label=aminoglycoside-3-phosphotransferase
                     /protein_id="ABB00290.1"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPHAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHNLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     rep_origin      1222..2009
                     /label=OriE
                     /note="OriE"
     rep_origin      1267..1855
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     rep_origin      2014..3857
                     /note="contains attP and int; similar to mycobacteriophage 
                     L5"
     CDS             2697..3695
                     /codon_start=1
                     /product="integrase"
                     /label=integrase
                     /note="derived from mycobacteriophage L5"
                     /protein_id="ABB00291.1"
                     /translation="MDAEAWLAGEKRLIEMETWTPPQDRAKKAAASAITLEEYTRKWLV
                     ERDLADGTRDLYSGHAERRIYPVLGEVAVTEMTPALVRAWWAGMGRKHPTARRHAYNVL
                     RAVMNTAVEDKLIAENPCRIEQKAADERDVEALTPEELDIVAAEIFEHYRIAAYILAWT
                     SLRFGELIELRRKDIVDDGMTMKLRVRRGASRVGNKIVVGNAKTVRSKRPVTVPPHVAE
                     MIRAHMKDRTKMNKGPEAFLVTTTQGNRLSKSAFTKSLKRGYAKIGRPELRIHDLRAVG
                     ATFAAQAGATTKELMARLGHTTPRMAMKYQMASEARDEAIAEAMSKLAKTS"
     terminator      3917..3963
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     regulatory      3920..3963
                     /regulatory_class="terminator"
                     /note="rrnBT1"