LOCUS       Exported                9994 bp ds-DNA     circular SYN 13-MAY-2021
DEFINITION  For packaging hDot1Lwt to retrovirus..
ACCESSION   .
VERSION     .
KEYWORDS    MSCB-hDot1Lwt
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9994)
  AUTHORS   Okada Y, Feng Q, Lin Y, Jiang Q, Li Y, Coffield VM, Su L, Xu G, 
            Zhang Y
  TITLE     hDOT1L links histone methylation to leukemogenesis.
  JOURNAL   Cell. 2005 Apr 22;121(2):167-78.
  PUBMED    15851025
REFERENCE   2  (bases 1 to 9994)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell."; 
            date: "2005-04-22"; volume: "121(2)"; pages: "167-78"
FEATURES             Location/Qualifiers
     source          1..9994
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     CDS             1..417
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     347..369
                     /label=pLXSN 5
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     385..401
                     /label=pBABE 5
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     CDS             428..454
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     promoter        5344..5843
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(5379..5402)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     5776..5795
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     LTR             5914..6428
                     /label=3 LTR
                     /note="3 long terminal repeat from murine embryonic stem 
                     cell virus"
     protein_bind    6590..6606
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(6614..6644)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6659..6680
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6797..6814)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6968..7556)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7048..7067)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(7727..8587)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8350..8369
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8588..8692)
                     /gene="bla"
                     /label=AmpR promoter
     enhancer        8849..9152
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        9153..9356
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     9307..9327
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     misc_feature    9595..9794
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"