LOCUS       Exported                5730 bp ds-DNA     circular SYN 17-DEC-2018
DEFINITION  Staphylococcal shuttle vector pCN55, complete sequence.
ACCESSION   KR781474
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5730)
  AUTHORS   Riojas MA, Bojja K, Byrd R, Hamid S, Mooney S, Monaco A, Alalade R, 
            King S, Hazbon MH.
  TITLE     Sequencing of a set of cassette-based shuttle vectors for 
            Gram-positive bacteria deposited at BEI resources from the NARSA 
            collection
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5730)
  AUTHORS   Riojas MA, Bojja K, Byrd R, Hamid S, Mooney S, Monaco A, Alalade R, 
            King S, Hazbon MH.
  TITLE     Direct Submission
  JOURNAL   Submitted (14-MAY-2015) BEI Bacteriology, BEI Resources/American 
            Type and Culture Collection (ATCC), 10801 University Boulevard, 
            Manassas, VA 20110-2209, USA
REFERENCE   3  (bases 1 to 5730)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Assembly Method       :: CLC Genomics Workbench v. 7.0.2
            Coverage              :: 52846
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5730
                     /organism="Staphylococcal shuttle vector pCN55"
                     /mol_type="other DNA"
                     /clone="NRS626"
                     /note="maintained in Escherichia coli grown in media 
                     supplemented with 100 ug/ml ampicillin"
                     /db_xref="taxon:1740136"
                     /culture_collection="BEI:NARSA NR-46166"
     misc_feature    1..2089
                     /note="pT181copwt repC; Staphylococcus replication 
                     cassette; derived from J04764"
     misc_feature    159..392
                     /label=RS-B site
                     /note="RS-B site"
     rep_origin      656..823
                     /note="ori; derived from Staphylococcus aureus"
     CDS             688..1632
                     /codon_start=1
                     /product="RepC"
                     /label=RepC
                     /note="polypeptide A"
                     /protein_id="AMR43712.1"
                     /translation="MYKNNHANHSNHLENHDLDNFSKTGYSNSRLDAHTVCISDPKLSF
                     DAMTIVGNLNRDNAQALSKFMSVEPQIRLWDILQTKFKAKALQEKVYIEYDKVKADSWD
                     RRNMRIEFNPNKLTRDEMIWLKQNIISYMEDDGFTRLDLAFDFEDDLSDYYAMSDKAVK
                     KTIFYGRNGKPETKYFGVRDSNRFIRIYNKKQERKDNADAEVMSEHLWRVEIELKRDMV
                     DYWNDCFSDLHILQPDWKTIQRTADRAIVFMLLSDEEEWGKLHRNSRTKYKNLIKEISP
                     VDLTDLMKSTLKANEKQLQKQIDFWQHEFKFWK"
     variation       1132..1134
                     /label=E149D
                     /note="E149D"
     misc_feature    1794..2089
                     /label=truncated polypeptide B
                     /note="truncated polypeptide B"
     misc_feature    2104..3344
                     /note="Tn554 aad9; involved in spectinomycin resistance; 
                     derived from X02588"
     regulatory      2246..2251
                     /regulatory_class="minus_35_signal"
     regulatory      2269..2274
                     /regulatory_class="minus_10_signal"
     precursor_RNA   2290..3332
                     /product="spc transcript"
     regulatory      2423..2428
                     /regulatory_class="ribosome_binding_site"
     CDS             2434..3216
                     /codon_start=1
                     /product="streptomycin 3-adenylyltransferase"
                     /label=streptomycin 3-adenylyltransferase
                     /note="involved in aminoglycoside resistance"
                     /protein_id="AMR43711.1"
                     /translation="MSNLINGKIPNQAIQTLKIVKDLFGSSIVGVYLFGSAVNGGLRIN
                     SDVDVLVVVNHSLPQLTRKKLTERLMTISGKIGNTDSVRPLEVTVINRSEVVPWQYPPK
                     REFIYGEWLRGEFENGQIQEPSYDPDLAIVLAQARKNSISLFGPDSSSILVSVPLTDIR
                     RAIKDSLPELIEGIKGDERNVILTLARMWQTVTTGEITSKDVAAEWAIPLLPKEHVTLL
                     DIARKGYRGECDDKWEGLYSKVKALVKYMKNSIETSLN"
     regulatory      3292..3338
                     /regulatory_class="terminator"
     repeat_region   3292..3309
                     /label=inverted repeat
                     /note="inverted repeat"
     repeat_region   3319..3338
                     /label=inverted repeat
                     /note="inverted repeat"
     misc_feature    3418..5511
                     /note="amp ColE1 ori; Escherichia coli replication and 
                     selection cassette; derived from L09137"
     promoter        3666..3770
                     /gene="bla"
                     /label=AmpR promoter
     CDS             3771..4631
                     /codon_start=1
                     /product="beta lactamase"
                     /label=beta lactamase
                     /note="involved in ampicillin resistance"
                     /protein_id="AMR43713.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      4802..5390
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     rep_origin      complement(5006..5286)
                     /direction=LEFT
                     /note="ori; derived from Escherichia coli"
     misc_feature    5520..3
                     /label=MCS-pUC19
                     /note="MCS-pUC19"
     misc_feature    5527..5571
                     /note="polylinker; derived from M13mp19"
     misc_feature    5572..5729
                     /label=lac operon
                     /note="lac operon"
     primer_bind     complement(5572..5588)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"