LOCUS       Exported                4050 bp ds-DNA     circular SYN 17-DEC-2018
DEFINITION  Expression vector pCMVTNT(TM), complete sequence.
ACCESSION   AF477200
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4050)
  AUTHORS   English J.
  TITLE     pCMVTNT(TM) Vector Technical Bulletin (TB305)
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4050)
  AUTHORS   English J.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2002) Technical Writing, Promega Corporation, 2800
            Woods Hollow Road, Madison, WI 53711-5399, USA
REFERENCE   3  (bases 1 to 4050)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..4050
                     /organism="Expression vector pCMVTNT(TM)"
                     /mol_type="genomic DNA"
                     /note="designed for expressing cloned genes in vivo or in 
                     vitro"
                     /db_xref="taxon:187377"
     regulatory      1..795
                     /regulatory_class="promoter"
                     /note="cytomegalovirus (CMV) immediate-early 
                     enhancer/promoter region"
     enhancer        138..517
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        518..721
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     intron          857..989
                     /note="chimeric"
     regulatory      1034..1052
                     /regulatory_class="promoter"
                     /note="T7 RNA polymerase promoter"
     promoter        1034..1052
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        1058..1076
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     regulatory      1058..1074
                     /regulatory_class="promoter"
                     /note="SP6 RNA polymerase promoter"
     misc_feature    1075..1101
                     /label=5 beta globin leader sequence
                     /note="5 beta globin leader sequence"
     misc_feature    1102..1148
                     /label=multiple cloning region
                     /note="multiple cloning region"
     misc_feature    1155..1376
                     /label=SV40 late polyadenylation signal
                     /note="SV40 late polyadenylation signal"
     polyA_signal    1164..1285
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     misc_feature    1466..1921
                     /label=phage f1 region
                     /note="phage f1 region"
     rep_origin      1466..1921
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        2253..2357
                     /gene="bla"
                     /label=AmpR promoter
     CDS             2358..3218
                     /codon_start=1
                     /product="AmpR"
                     /label=AmpR
                     /note="beta-lactamase"
                     /protein_id="AAL83302.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      3389..3977
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"