LOCUS       Exported                4319 bp ds-DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector pCMVTAG4a, complete sequence.
ACCESSION   AF073000
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4319)
  AUTHORS   Lu Q.
  TITLE     pCMVTAG4a
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4319)
  AUTHORS   Lu Q.
  TITLE     Direct Submission
  JOURNAL   Submitted (22-JUN-1998) Technical Services, Stratagene, 11011 N. 
            Torrey Pines Rd., San Diego, CA 92037, USA
REFERENCE   3  (bases 1 to 4319)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..4319
                     /organism="Cloning vector pCMVTAG4a"
                     /lab_host="Escherichia coli K12"
                     /mol_type="genomic DNA"
                     /db_xref="taxon:78252"
     regulatory      1..602
                     /regulatory_class="promoter"
                     /note="CMV promoter"
     enhancer        67..370
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        371..574
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     promoter        620..638
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     misc_feature    656..738
                     /label=multiple cloning site
                     /note="multiple cloning site"
     primer_bind     complement(725..741)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             744..767
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     promoter        complement(821..839)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    852..1235
                     /label=SV40 polyA signal region
                     /note="SV40 polyA signal region"
     polyA_signal    1113..1234
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      1235..1696
                     /label=f1 origin of replication
                     /note="f1 origin of replication"
     rep_origin      complement(1241..1696)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     regulatory      1697..1828
                     /regulatory_class="promoter"
                     /note="Pbla promoter"
     promoter        1723..1827
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      1829..2220
                     /label=SV40 origin of replication
                     /note="SV40 origin of replication"
     promoter        1829..2186
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      2037..2172
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             2221..3015
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /function="kanamycin resistance"
                     /label=aminoglycoside phosphotransferase
                     /protein_id="AAC25429.1"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     polyA_signal    3247..3294
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      3462..4309
                     /label=ColE1 origin of replication
                     /note="ColE1 origin of replication"
     rep_origin      3623..4211
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"