LOCUS       Exported                4323 bp ds-DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector pCMVTAG2a, complete sequence.
ACCESSION   AF072538
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4323)
  AUTHORS   Lu Q.
  TITLE     pCMVTAG2a
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4323)
  AUTHORS   Lu Q.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JUN-1998) Technical Services, Stratagene, 11011 N. 
            Torrey Pines Rd., San Diego, CA 92037, USA
REFERENCE   3  (bases 1 to 4323)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..4323
                     /organism="Cloning vector pCMVTAG2a"
                     /lab_host="Escherichia coli K12"
                     /mol_type="genomic DNA"
                     /db_xref="taxon:78246"
     regulatory      1..602
                     /regulatory_class="promoter"
                     /note="CMV promoter"
     enhancer        67..370
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        371..574
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     promoter        620..638
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     regulatory      673..682
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     CDS             682..705
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     misc_feature    709..779
                     /label=multiple cloning site
                     /note="multiple cloning site"
     primer_bind     complement(752..768)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        complement(825..843)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    856..1239
                     /label=SV40 polyA signal region
                     /note="SV40 polyA signal region"
     polyA_signal    1117..1238
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      1239..1700
                     /label=f1 origin of replication
                     /note="f1 origin of replication"
     rep_origin      complement(1245..1700)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     regulatory      1701..1832
                     /regulatory_class="promoter"
                     /note="Pbla promoter"
     promoter        1727..1831
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      1833..2224
                     /label=SV40 origin of replication
                     /note="SV40 origin of replication"
     promoter        1833..2190
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      2041..2176
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             2225..3019
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /function="kanamycin resistance"
                     /label=aminoglycoside phosphotransferase
                     /protein_id="AAC25420.1"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     polyA_signal    3251..3298
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      3466..4313
                     /label=ColE1 origin of replication
                     /note="ColE1 origin of replication"
     rep_origin      3627..4215
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"