LOCUS       Exported                4707 bp ds-DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector PCMV6-XL4, complete sequence.
ACCESSION   AF067196
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4707)
  AUTHORS   Li X.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-1998) Origene Technologies Inc., 13 Taft Ct., 
            Rockville, MD 20850, USA
REFERENCE   2  (bases 1 to 4707)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..4707
                     /organism="Cloning vector PCMV6-XL4"
                     /host="Escherichia coli"
                     /mol_type="genomic DNA"
                     /db_xref="taxon:77618"
     primer_bind     166..182
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     regulatory      201..926
                     /regulatory_class="promoter"
                     /note="CMV"
     enhancer        343..722
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        723..926
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     promoter        952..970
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      953..971
                     /regulatory_class="promoter"
                     /note="T7 promoter"
     misc_feature    972..1030
                     /label=multiple cloning site
                     /note="multiple cloning site"
     primer_bind     1031..1048
                     /label=M13 reverse primer
                     /note="M13 reverse primer"
     primer_bind     complement(1032..1048)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     polyA_signal    1062..1684
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     regulatory      1063..1684
                     /regulatory_class="polyA_signal_sequence"
                     /note="human growth hormone"
     promoter        1713..2042
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      1740..2047
                     /label=SV40 origin
                     /note="SV40 origin"
     rep_origin      1893..2028
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        complement(2081..2099)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2113..2129)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    2137..2153
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(2161..2191)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2206..2227
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      2457..3129
                     /label=ColE1
                     /note="ColE1"
     rep_origin      complement(2515..3103)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3274..4134)
                     /codon_start=1
                     /product="ampicillin resistance protein"
                     /label=ampicillin resistance protein
                     /protein_id="AAC24837.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(4135..4239)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      4266..4694
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     rep_origin      4267..4679
                     /label=f1
                     /note="f1"