LOCUS       Exported                4657 bp ds-DNA     circular SYN 17-DEC-2018
DEFINITION  Eukaryotic expression vector pCMV5, complete sequence.
ACCESSION   AF239249
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4657)
  AUTHORS   Andersson S, Davis DL, Dahlback H, Jornvall H, Russell DW.
  TITLE     Cloning, structure, and expression of the mitochondrial cytochrome 
            P-450 sterol 26-hydroxylase, a bile acid biosynthetic enzyme
  JOURNAL   J. Biol. Chem. 264 (14), 8222-8229 (1989)
  PUBMED    2722778
REFERENCE   2  (bases 1 to 4657)
  AUTHORS   Andersson S, Davis DL, Dahlback H, Jornvall H, Russell DW.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-FEB-2000) Molecular Genetics, University of Texas 
            Southwestern Medical Center at Dallas, 5323 Harry Hines Blvd., 
            Dallas, TX 75235, USA
REFERENCE   3  (bases 1 to 4657)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..4657
                     /organism="Expression vector pCMV5"
                     /mol_type="genomic DNA"
                     /db_xref="taxon:120120"
     primer_bind     142..158
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     enhancer        319..698
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        699..902
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     polyA_signal    987..1463
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     promoter        1636..1966
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      1817..1952
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        complement(2005..2023)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2038..2054)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    2062..2078
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(2086..2116)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2131..2152
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(2439..3027)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3198..4058)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(4059..4163)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      4190..4618
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"