LOCUS       Exported                8964 bp ds-DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector pCMH500, complete sequence.
ACCESSION   AY768816
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8964)
  AUTHORS   Strive T, Hardy CM, French N, Wright JD, Nagaraja N, Reubel GH.
  TITLE     Development of canine herpesvirus based antifertility vaccines for 
            foxes using bacterial artificial chromosomes
  JOURNAL   Vaccine 24 (7), 980-988 (2006)
  PUBMED    16198458
REFERENCE   2  (bases 1 to 8964)
  AUTHORS   Hardy CM.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-SEP-2004) Sustainable Ecosystems, CSIRO, GPO Box 284, 
            Canberra, ACT 2601, Australia
REFERENCE   3  (bases 1 to 8964)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..8964
                     /organism="Cloning vector pCMH500"
                     /focus
                     /mol_type="other DNA"
                     /db_xref="taxon:314049"
     source          1..6089
                     /organism="Canid herpesvirus 1"
                     /isolate="greyhound"
                     /mol_type="other DNA"
                     /country="Australia"
                     /db_xref="taxon:170325"
     misc_feature    1..1095
                     /label=similar to UL25
                     /note="similar to UL25"
     CDS             complement(1153..1935)
                     /codon_start=1
                     /product="UL24"
                     /label=UL24
                     /protein_id="AAX18922.1"
                     /translation="MKRNQRQTIRTRLCAGIRCHNRFYSVLTRDVKLASQNGIYSDRLS
                     LLFGDLIPADILKRAGSVNLAFEVNLGQRRPDCVCTINLEHESMGLCILIELKTCRFSK
                     NMNTASKTTQQRGGLKQLNDSVSLITKILPPGDGKVVLIPLLVFIAQRGMNILKVTSLP
                     HKQLSSNIKTLAANLTKLAEYIPKPAKPVKGSNNRNKSLKQKVNCLKCNFVNCICIKTS
                     NVEFINLTNTEKSTTCSNFESSTLLNPVKLISSLFNKD"
     misc_feature    1947..2460
                     /label=similar to UL23 thymidine kinase
                     /note="similar to UL23 thymidine kinase"
     misc_feature    2268..2275
                     /note="NotI cloning site for insertion of transgenes; 
                     disrupts UL23 sequence"
     CDS             2570..4960
                     /codon_start=1
                     /product="UL22"
                     /label=UL22
                     /note="glycoprotein H"
                     /protein_id="AAX18923.1"
                     /translation="MEVKYNFITFIFVIFFMLVFSNDLDNGEYKNLSLSNDFSYSSNVN
                     ITTIENLFSYNNKFVFFFIFNKDSKRENGNLFLFPNFIFKSNKTENNYNIKSQPSEKQL
                     TVFFNPYINLYLSSKYLIDFGVIPESSIQDWYFPRSIQTSTEQNPFGFILSPKRHVPNN
                     GFFNKHKNLYKDGLLNDIMYVNLNSFSIPRKTYIPIYESTWPFDISLFESTLIQNENFC
                     INITIGKEFMGMSITSSKYPPLEMITTPHDANVEMITRYKASMMLDPPGPSEGPLYKVF
                     IIGYGTSKTDSNMYKIMQTIASYPEESLDYRYHLSMANFEVCFLLTYERKFLTNKMDME
                     YFYKIFSRLSTALFSLSEMVRLSKYILNDEIIDIDFNINILTNIIVNHKMFKLSKQLIT
                     SSQVNILLESSRENIVNNLNTFENYNLIFKNDELKIKYLKAVYTYLEIPTEKSLIISRD
                     IINTLYNNSLYETIDWNVTSRKSLFLATSLLLLKTQNIVELRKIILQCTSMCTSDHAIS
                     IEWSLENILNAKNNFKKQFSITDMFSPCMTSTRYDLIEDLHIVDLISVIPNNGNFKNLF
                     SMGHKAASVVNSNILFGSNKIKMLIPELFICKIDINFKFLAILPLNNNNSYIITRKILN
                     RGLTYNVNGIDIANPIFISYINSSNCLYSSVEILPLNLINPKNSKECLYCGSVIMRYLS
                     SGVIIDLLYINDKEAETQLAAGVNSTIPSFNPLSYPVNLKTLLLFPNGTIVTINSITSH
                     EQIKFSNTFIVISIVGIIITSFVVYGIFKMLCSFSYVKYSLLK"
     misc_feature    5003..5420
                     /label=GC-rich region
                     /note="GC-rich region"
     misc_feature    5576..6089
                     /label=similar to UL21
                     /note="similar to UL21"
     misc_feature    6090..8964
                     /label=pBluescript KS- cloning vector sequences
                     /note="pBluescript KS- cloning vector sequences"
     promoter        complement(6107..6125)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6132..6148)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      complement(6289..6744)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        6771..6875
                     /gene="bla"
                     /label=AmpR promoter
     CDS             6876..7736
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      7907..8495
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    8783..8804
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        8819..8849
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8857..8873
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8881..8897
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        8918..8936
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(8954..6)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"