LOCUS       Exported                3551 bp ds-DNA     circular SYN 17-DEC-2018
DEFINITION  Expression vector pCMH411, complete sequence.
ACCESSION   AY122058
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3551)
  AUTHORS   Hardy CM.
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2002) Wildlife, Pests and Diseases, CSIRO 
            Sustainable Ecosystems, GPO Box 284, Canberra, ACT 2601, Australia
REFERENCE   2  (bases 1 to 3551)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..3551
                     /organism="Expression vector pCMH411"
                     /mol_type="genomic DNA"
                     /db_xref="taxon:202450"
     regulatory      66..425
                     /regulatory_class="promoter"
                     /note="from Human cytomegalovirus immediate early 1 (IE-1) 
                     gene"
     promoter        149..351
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     misc_feature    437..456
                     /label=multiple cloning site
                     /note="multiple cloning site"
     regulatory      457..583
                     /regulatory_class="terminator"
                     /note="Simian virus 40 derived polyA terminator"
     regulatory      602..611
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     promoter        complement(642..660)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(667..683)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      824..1279
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        1360..1464
                     /gene="bla"
                     /label=AmpR promoter
     CDS             1465..2325
                     /codon_start=1
                     /product="beta lactamase"
                     /function="ampicillin resistance"
                     /label=beta lactamase
                     /protein_id="AAM82245.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCHTLLSRIDAGQEQLGRRARYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMPTTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      2496..3084
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    3372..3393
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        3408..3438
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3446..3462
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     3470..3486
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        3504..3522
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"