LOCUS       Exported                3734 bp ds-DNA     circular SYN 17-DEC-2018
DEFINITION  Expression vector pCMH148, complete sequence.
ACCESSION   AY122059
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3734)
  AUTHORS   Hardy CM.
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2002) Wildlife, Pests and Diseases, CSIRO 
            Sustainable Ecosystems, GPO Box 284, Canberra, ACT 2601, Australia
REFERENCE   2  (bases 1 to 3734)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..3734
                     /organism="Expression vector pCMH148"
                     /mol_type="genomic DNA"
                     /note="derived from expression vector pCMVbeta presented in
                     GenBank Accession Number U02451"
                     /db_xref="taxon:202451"
     regulatory      38..562
                     /regulatory_class="promoter"
                     /note="from Human cytomegalovirus immediate early 1 (IE-1) 
                     gene"
     enhancer        40..343
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        344..547
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     intron          685..783
                     /note="modified SV40 intron"
     intron          685..781
                     /label=SV40 intron
                     /note="modified SV40 intron with splice donor and acceptor 
                     sites"
     misc_feature    835..858
                     /label=multiple cloning site
                     /note="multiple cloning site"
     regulatory      859..1056
                     /regulatory_class="terminator"
                     /note="Simian virus 40 derived polyA terminator"
     polyA_signal    860..994
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1118..1134)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    1142..1158
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(1166..1196)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1211..1232
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(1520..2108)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2279..3139)
                     /codon_start=1
                     /product="beta lactamase"
                     /function="ampicillin resistance"
                     /label=beta lactamase
                     /protein_id="AAM82246.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(3140..3244)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     3718..3734
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"