LOCUS       Exported                7187 bp ds-DNA     circular SYN 17-DEC-2018
DEFINITION  Mammalian expression vector pCMF2E-mCASP-8, complete sequence.
ACCESSION   LT727118
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7187)
  AUTHORS   De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman 
            Fonseca M, Vanhoucke M, Beyaert R.
  TITLE     BCCM/LMBP Plasmid collection
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7187)
  AUTHORS   De Schamphelaire W.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2017) BCCM/LMBP, Universiteit Gent, 
            Technologiepark 927, 9052, BELGIUM
REFERENCE   3  (bases 1 to 7187)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..7187
                     /organism="Mammalian expression vector pCMF2E-mCASP-8"
                     /mol_type="other DNA"
                     /note="BCCM/LMBP Plasmid collection (Ghent 
                     University,Belgium) accession number LMBP 4589."
                     /db_xref="taxon:1944956"
     CDS             13..54
                     /codon_start=1
                     /note="unnamed protein product; v-src 
                     myristoylation-targeting sequence"
                     /protein_id="SJL87493.1"
                     /translation="MGSSKSKPKDPSQR"
     CDS             61..381
                     /codon_start=1
                     /note="unnamed protein product; hFKBP1A"
                     /protein_id="SJL87494.1"
                     /translation="GVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKFDSSRDRNKP
                     FKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVELL
                     KLE"
     CDS             388..708
                     /codon_start=1
                     /note="unnamed protein product; hFKBP1A"
                     /protein_id="SJL87495.1"
                     /translation="GVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKFDSSRDRNKP
                     FKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVELL
                     KLE"
     CDS             715..2151
                     /codon_start=1
                     /note="unnamed protein product; mCASP-8"
                     /protein_id="SJL87496.1"
                     /translation="DFQSCLYAIAEELGSEDLAALKFLCLDYIPHKKQETIEDAQKLFL
                     RLREKGMLEEGNLSFLKELLFHISRWDLLVNFLDCNREEMVRELRDPDNVQISPYRVML
                     FKLSEEVSELELRSFKFLLNNEIPKCKLEDDLSLLEIFVEMEKRTMLAENNLETLKSIC
                     DQVNKSLLGKIEDYERSSTERRMSLEGREELPPSVLDEMSLKMAELCDSPREQDSESRT
                     SDKVYQMKNKPRGYCLIINNHDFSKAREDITQLRKMKDRKGTDCDKEALSKTFKELHFE
                     IVSYDDCTANEIHEILEGYQSADHKNKDCFICCILSHGDKGVVYGTDGKEASIYDLTSY
                     FTGSKCPSLSGKPKIFFIQACQGSNFQKGVPDEAGFEQQNHTLEVDSSSHKNYIPDEAD
                     FLLGMATVKNCVSYRDPVNGTWYIQSLCQSLRERCPQGDDILSILTGVNYDVSNKDDRR
                     NKGKQMPQPTFTLRKKLFFPP"
     misc_feature    2158..2184
                     /label=HA epitope
                     /note="HA epitope"
     CDS             2158..2184
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     intron          2216..2788
                     /label=-beta-globin intron
                     /note="intron from rabbit beta-globin gene"
     regulatory      2918..3009
                     /regulatory_class="terminator"
                     /note="beta-globin polyA"
     polyA_site      2985..3040
                     /label=-beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal"
     misc_feature    3010..3364
                     /label=3 FR of beta-globin
                     /note="3 FR of beta-globin"
     rep_origin      3393..3528
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     rep_origin      complement(3404..3479)
                     /direction=LEFT
                     /label=SV40 ori
                     /note="SV40 ori"
     rep_origin      complement(3621..4023)
                     /direction=LEFT
                     /label=pMB1 ori
                     /note="pMB1 ori"
     rep_origin      complement(3805..4393)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(4564..5436)
                     /codon_start=1
                     /note="unnamed protein product; ampicillin"
                     /protein_id="SJL87497.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFQVEVAMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDL
                     VEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTR
                     LDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAE
                     IGASLIKHW"
     promoter        complement(5437..5541)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(5873..6328)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 ori"
     primer_bind     6473..6489
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        6496..6514
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      6496..6512
                     /regulatory_class="promoter"
                     /note="T7 promoter"
     regulatory      6533..7040
                     /regulatory_class="promoter"
                     /note="hCMV-IE promoter and enhancer"
     enhancer        6533..6836
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        6837..7040
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"