LOCUS       Exported                7106 bp ds-DNA     circular SYN 17-DEC-2018
DEFINITION  Mammalian expression vector pCMF2E-mCASP-2, complete sequence.
ACCESSION   LT727117
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7106)
  AUTHORS   De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman 
            Fonseca M, Vanhoucke M, Beyaert R.
  TITLE     BCCM/LMBP Plasmid collection
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7106)
  AUTHORS   De Schamphelaire W.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2017) BCCM/LMBP, Universiteit Gent, 
            Technologiepark 927, 9052, BELGIUM
REFERENCE   3  (bases 1 to 7106)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..7106
                     /organism="Mammalian expression vector pCMF2E-mCASP-2"
                     /mol_type="other DNA"
                     /note="BCCM/LMBP Plasmid collection (Ghent 
                     University,Belgium) accession number LMBP 4588."
                     /db_xref="taxon:1944954"
     CDS             13..54
                     /codon_start=1
                     /note="unnamed protein product; v-src 
                     myristoylation-targeting sequence"
                     /protein_id="SJL87488.1"
                     /translation="MGSSKSKPKDPSQR"
     CDS             61..381
                     /codon_start=1
                     /note="unnamed protein product; hFKBP1A"
                     /protein_id="SJL87489.1"
                     /translation="GVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKFDSSRDRNKP
                     FKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVELL
                     KLE"
     CDS             388..708
                     /codon_start=1
                     /note="unnamed protein product; hFKBP1A"
                     /protein_id="SJL87490.1"
                     /translation="GVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKFDSSRDRNKP
                     FKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVELL
                     KLE"
     CDS             715..2070
                     /codon_start=1
                     /note="unnamed protein product; mCASP-2"
                     /protein_id="SJL87491.1"
                     /translation="MAAPSGRSQSSLHRKGLMAADRRSRILAVCGMHPDHQETLKKNRV
                     VLAKQLLLSELLEHLLEKDIITLEMRELIQAKGGSFSQNVELLNLLPKRGPQAFDAFCE
                     ALRETRQGHLEDLLLTTLSDIQHVLPPLSCDYDTSLPFSVCESCPPHKQLRLSTDATEH
                     SLDNGDGPPCLLVKPCTPEFYQAHYQLAYRLQSQPRGLALVLSNVHFTGEKDLEFRSGG
                     DVDHTTLVTLFKLLGYNVHVLHDQTAQEMQEKLQNFAQLPAHRVTDSCVVALLSHGVEG
                     GIYGVDGKLLQLQEVFRLFDNANCPSLQNKPKMFFIQACRGDETDRGVDQQDGKNHTQS
                     PGCEESDAGKEELMKMRLPTRSDMICGYACLKGNAAMRNTKRGSWYIEALTQVFSERAC
                     DMHVADMLVKVNALIKEREGYAPGTEFHRCKEMSEYCSTLCQQLYLFPGYPPT"
     misc_feature    2077..2103
                     /label=HA epitope
                     /note="HA epitope"
     CDS             2077..2103
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     intron          2135..2707
                     /label=-beta-globin intron
                     /note="intron from rabbit beta-globin gene"
     regulatory      2837..2928
                     /regulatory_class="terminator"
                     /note="beta-globin polyA"
     polyA_site      2904..2959
                     /label=-beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal"
     misc_feature    2929..3283
                     /label=3 FR of beta-globin
                     /note="3 FR of beta-globin"
     rep_origin      3312..3447
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     rep_origin      complement(3323..3398)
                     /direction=LEFT
                     /label=SV40 ori
                     /note="SV40 ori"
     rep_origin      complement(3540..3942)
                     /direction=LEFT
                     /label=pMB1 ori
                     /note="pMB1 ori"
     rep_origin      complement(3724..4312)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(4483..5355)
                     /codon_start=1
                     /note="unnamed protein product; ampicillin"
                     /protein_id="SJL87492.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFQVEVAMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDL
                     VEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTR
                     LDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAE
                     IGASLIKHW"
     promoter        complement(5356..5460)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(5792..6247)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 ori"
     primer_bind     6392..6408
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        6415..6433
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      6415..6431
                     /regulatory_class="promoter"
                     /note="T7 promoter"
     regulatory      6452..6959
                     /regulatory_class="promoter"
                     /note="hCMV-IE promoter and enhancer"
     enhancer        6452..6755
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        6756..6959
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"