LOCUS       Exported                3707 bp ds-DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector pCloneNat1, complete sequence.
ACCESSION   EF101285
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3707)
  AUTHORS   Gregan J, Rabitsch KP, Rumpf C, Nasmyth K.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2006) Chromosome Biology, University of Vienna, 
            Dr. Bohrgasse 1, Vienna 1030, Austria
REFERENCE   2  (bases 1 to 3707)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..3707
                     /organism="Cloning vector pCloneNat1"
                     /mol_type="other DNA"
                     /note="constructed by digestion with HindIII and BamHI of 
                     pAG25 (Goldstein and McCusker, 1999) and ligation of two 
                     annealed oligonucleotides coding for new restriction sites.
                     The resulting MCS contains the following restriction sites:
                     HindIII-XhoI-SspBI-NcoI-BamHI-BglII"
                     /db_xref="taxon:413948"
     gene            115..1237
                     /label=natMX6
                     /note="yeast selectable marker conferring nourseothricin 
                     resistance"
     promoter        115..458
                     /label=TEF promoter
                     /note="Ashbya gossypii TEF promoter"
     CDS             459..1034
                     /codon_start=1
                     /gene="natMX4"
                     /product="nourseothricin N-acetyltransferase"
                     /label=natMX4
                     /note="confers resistance to Clonat"
                     /protein_id="ABL09005.1"
                     /translation="MGTTLDDTAYRYRTSVPGDAEAIEALDGSFTTDTVFRVTATGDGF
                     TLREVPVDPPLTKVFPDDESDDESDDGEDGDPDSRTFVAYGDDGDLAGFVVISYSAWNR
                     RLTVEDIEVAPEHRGHGVGRALMGLATEFAGERGAGHLWLEVTNVNAPAIHAYRRMGFT
                     LCGLDTALYDGTASDGERQALYMSMPCP"
     gene            459..1034
                     /gene="natMX4"
                     /label=natMX4
     terminator      1040..1237
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
     promoter        complement(1345..1363)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      complement(1621..2209)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2380..3240)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(3241..3345)
                     /gene="bla"
                     /label=AmpR promoter
     promoter        3691..2
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"