LOCUS       Exported                4160 bp ds-DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector pCloneHyg1, complete sequence.
ACCESSION   EF101286
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4160)
  AUTHORS   Gregan J, Rabitsch KP, Rumpf C, Nasmyth K.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2006) Chromosome Biology, University of Vienna, 
            Dr. Bohrgasse 1, Vienna 1030, Austria
REFERENCE   2  (bases 1 to 4160)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..4160
                     /organism="Cloning vector pCloneHyg1"
                     /mol_type="other DNA"
                     /label=based on pAG32 from Goldstein and McCusker, 1999
                     /note="based on pAG32 from Goldstein and McCusker, 1999"
                     /db_xref="taxon:413947"
     gene            115..1690
                     /label=hphMX6
                     /note="yeast selectable marker conferring hygromycin 
                     resistance"
     promoter        115..458
                     /label=TEF promoter
                     /note="Ashbya gossypii TEF promoter"
     CDS             459..1487
                     /codon_start=1
                     /gene="hphMX4"
                     /product="hygromycin B phosphotransferase"
                     /label=hphMX4
                     /note="confers resistance to hygromycin B"
                     /protein_id="ABL09006.1"
                     /translation="MGKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGR
                     GYVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDL
                     PETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHW
                     QTVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAM
                     FGDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVD
                     GNFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRA
                     KE"
     gene            459..1487
                     /gene="hphMX4"
                     /label=hphMX4
     terminator      1493..1690
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
     promoter        complement(1798..1816)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      complement(2074..2662)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2833..3693)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(3694..3798)
                     /gene="bla"
                     /label=AmpR promoter
     promoter        4144..2
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"