LOCUS       Exported                3503 bp ds-DNA     circular SYN 17-DEC-2018
DEFINITION  Yeast knockout vector pCloneBle1, complete sequence.
ACCESSION   GQ354685
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3503)
  AUTHORS   Spirek M, Carnecka M, Gregan J.
  TITLE     New plasmids for high-throughput gene deletions in 
            Schizosaccharomyces pombe
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3503)
  AUTHORS   Spirek M, Carnecka M, Gregan J.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-JUL-2009) Chromosome Biology, University of Vienna, 
            Viehmarktgasse 2a, Wien 1030, Austria
REFERENCE   3  (bases 1 to 3503)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..3503
                     /organism="Yeast knockout vector pCloneBle1"
                     /mol_type="other DNA"
                     /db_xref="taxon:664750"
     gene            115..1036
                     /label=bleMX6
                     /note="yeast selectable marker conferring phleomycin 
                     resistance"
     promoter        115..458
                     /label=TEF promoter
                     /note="Ashbya gossypii TEF promoter"
     CDS             459..833
                     /codon_start=1
                     /gene="ble"
                     /product="phleomycin resistance protein"
                     /label=ble
                     /protein_id="ACU29449.1"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     gene            459..833
                     /gene="ble"
                     /label=ble
     terminator      839..1036
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
     promoter        complement(1141..1159)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      complement(1417..2005)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2176..3036)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=bla
                     /protein_id="ACU29450.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     gene            complement(2176..3036)
                     /gene="bla"
                     /label=bla
     promoter        complement(3037..3141)
                     /gene="bla"
                     /label=AmpR promoter
     promoter        3487..2
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"