LOCUS       Exported                3857 bp ds-DNA     circular SYN 14-DEC-2018
DEFINITION  Expression vector pCL311, complete sequence.
ACCESSION   EU391617
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3857)
  AUTHORS   Chen K, Li M, Lu F-P.
  TITLE     Construction of Bacillus subtilis expression vector pCL311
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3857)
  AUTHORS   Chen K, Li M, Lu F-P.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2008) College of Biotechnology, Tianjin University
            of Science & Technology, TEDA 13rd Street, TEDA District, Tanggu, 
            Tianjin 300457, China
REFERENCE   3  (bases 1 to 3857)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..3857
                     /organism="Expression vector pCL311"
                     /lab_host="Bacillus subtilis"
                     /mol_type="other DNA"
                     /db_xref="taxon:512389"
     CDS             343..1347
                     /codon_start=1
                     /product="replication protein"
                     /label=replication protein
                     /note="derived from Staphylococcus aureus"
                     /protein_id="ACB46141.1"
                     /translation="MGVSFNIMCPNSSIYSDEKSRVLVDKTKSGKVRPWREKKIANVDY
                     FELLHILEFKKAERVKDCAEILEYKQNRETGERKLYRVWFCKSRLCPMCNWRRAMKHGI
                     QSQKVVAEVIKQKPTVRWLFLTLTVKNVYDGEELNKSLSDMAQGFRRMMQYKKINKNLV
                     GFMRATEVTINNKDNSYNQHMHVLVCVEPTYFKNTENYVNQKQWIQFWKKAMKLDYDPN
                     VKVQMIRPKNKYKSDIQSAIDETAKYPVKDTDFMTDDEEKNLKRLSDLEEGLHRKRLIS
                     YGGLLKEIHKKLNLDDTEEGDLIHTDDDEKADEDGFSIIAMWNWERKNYFIKE"
     CDS             1516..2277
                     /codon_start=1
                     /product="neomycin resistance protein"
                     /label=neomycin resistance protein
                     /note="derived from Staphylococcus aureus"
                     /protein_id="ACB46140.1"
                     /translation="MNGPIIMTREERMKIVHEIKERILDKYGDDVKAIGVYGSLGRQTD
                     GPYSDIEMMCVMSTEEAEFSHEWTTGEWKVEVNFDSEEILLDYASQVESDWPLTHGQFF
                     SILPIYDSGGYLEKVYQTAKSVEAQTFHDAICALIVEELFEYAGKWRNIRVQGPTTFLP
                     SLTVQVAMAGAMLIGLHHRICYTTSASVLTEAVKQSDLPSGYDHLCQFVMSGQLSDSEK
                     LLESLENFWNGIQEWTERHGYIVDVSKRIPF"
     misc_feature    3747..3827
                     /note="alkaline protease signal peptide; derived from 
                     Bacillus alcalophillus PB92"