LOCUS       Exported                6624 bp ds-DNA     circular SYN 17-DEC-2018
DEFINITION  Single-copy integration vector pCL25, complete sequence.
ACCESSION   EF540894
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6624)
  AUTHORS   Luong TT, Lee CY.
  TITLE     Improved single-copy integration vectors for Staphylococcus aureus
  JOURNAL   J. Microbiol. Methods 70 (1), 186-190 (2007)
  PUBMED    17512993
REFERENCE   2  (bases 1 to 6624)
  AUTHORS   Luong TT, Lee CY.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-APR-2007) Microbiology and Immunology, Univ of 
            Arkansas for Medical Sciences, 4301 W. Markham St. Mail Slot 511, 
            Little Rock, AR 72205, USA
REFERENCE   3  (bases 1 to 6624)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..6624
                     /organism="Single-copy integration vector pCL25"
                     /mol_type="other DNA"
                     /db_xref="taxon:441911"
     misc_feature    1..57
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     CDS             complement(712..2091)
                     /codon_start=1
                     /gene="tetK"
                     /product="TetK"
                     /label=tetK
                     /note="tetracycline efflux protein"
                     /protein_id="ABQ23062.1"
                     /translation="MFSLYKKFKGLFYSVLFWLCILSFFSVLNEMVLNVSLPDIANHFN
                     TTPGITNWVNTAYMLTFSIGTAVYGKLSDYINIKKLLIIGISLSCLGSLIAFIGHNHFF
                     ILIFGRLVQGVGSAAFPSLIMVVVARNITRKKQGKAFGFIGSIVALGEGLGPSIGGIIA
                     HYIHWSYLLILPMITIVTIPFLIKVMVPGKSTKNTLDIVGIVLMSISIICFMLFTTNYN
                     WTFLILFTIFFVIFIKHISRVSNPFINPKLGKNIPFMLGLFSGGLIFSIVAGFISMVPY
                     MMKTIYHVNVATIGNSVIFPGTMSVIVFGYFGGFLVDRKGSLFVFILGSLSISISFLTI
                     AFFVEFSMWLTTFMFIFVMGGLSFTKTVISKIVSSSLSEEEVASGMSLLNFTSFLSEGT
                     GIAIVGGLLSLQLINRKLVLEFINYSSGVYSNILVAMAILIILCCLLTIIVFKRSEKQF
                     E"
     gene            complement(712..2091)
                     /gene="tetK"
                     /label=tetK
     CDS             complement(2880..3890)
                     /codon_start=1
                     /gene="aadA1"
                     /product="AadA1"
                     /label=aadA1
                     /note="aminoglycoside 3-adenylyltransferase"
                     /protein_id="ABQ23063.1"
                     /translation="MRSRNWSRTLTERSGGNGAVAVFMACYDCFFGVQSMPRASKQQAR
                     YAVGRCLMLWSSNDVTQQGSRPKTKLNIMREAVIAEVSTQLSEVVGVIERHLEPTLLAV
                     HLYGSAVDGGLKPHSDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTI
                     VVHDDIIPWRYPAKRELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPA
                     AEELFDPVPEQDLFEALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVA
                     ADWAMERLPAQYQPVILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     gene            complement(2880..3890)
                     /gene="aadA1"
                     /label=aadA1
     CDS             complement(2880..3671)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     CDS             complement(4923..5873)
                     /codon_start=1
                     /gene="repA"
                     /product="RepA"
                     /label=repA
                     /protein_id="ABQ23064.1"
                     /translation="MSELVVFKANELAISRYDLTEHETKLILCCVALLNPTIENPTRKE
                     RTVSFTYNQYAQMMNISRENAYGVLAKATRELMTRTVEIRNPLVKGFEIFQWTNYAKFS
                     SEKLELVFSEEILPYLFQLKKFIKYNLEHVKSFENKYSMRIYEWLLKELTQKKTHKANI
                     EISLDEFKFMLMLENNYHEFKRLNQWVLKPISKDLNTYSNMKLVVDKRGRPTDTLIFQV
                     ELDRQMDLVTELENNQIKMNGDKIPTTITSDSYLHNGLRKTLHDALTAKIQLTSFEAKF
                     LSDMQSKYDLNGSFSWLTQKQRTTLENILAKYGRI"
     gene            complement(4923..5873)
                     /gene="repA"
                     /label=repA
     rep_origin      5921..6143
                     /label=pSC101 ori
                     /note="low-copy replication origin that requires the Rep101
                     protein"