LOCUS       Exported                5435 bp ds-DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector pCint2, complete sequence.
ACCESSION   MH050934
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5435)
  AUTHORS   Zhang JY, Lin GM, Xing WY, Zhang CC.
  TITLE     Diversity of Growth Patterns Probed in Live Cyanobacterial Cells 
            Using a Fluorescent Analog of a Peptidoglycan Precursor
  JOURNAL   Front Microbiol 9, 791 (2018)
  PUBMED    29740419
REFERENCE   2  (bases 1 to 5435)
  AUTHORS   Zhang J-Y., Lin G-M., Xing W-Y., Zhang C-C.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2018) Institute of Hydrobiology, Donghu Nanlu, 
            Wuhan, Hubei 430072, 086
REFERENCE   3  (bases 1 to 5435)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..5435
                     /organism="Cloning vector pCint2"
                     /mol_type="other DNA"
                     /db_xref="taxon:2201264"
     CDS             complement(473..1894)
                     /codon_start=1
                     /gene="sacB"
                     /product="levansucrase"
                     /label=sacB
                     /note="derived from Bacillus subtilis"
                     /protein_id="AWK77089.1"
                     /translation="MNIKKFAKQATVLTFTTALLAGGATQAFAKETNQKPYKETYGISH
                     ITRHDMLQIPEQQKNEKYQVPEFDSSTIKNISSAKGLEVWDSWPLQNADGTVANYHGYH
                     IVFALAGDPKNADDTSIYMFYQKVGETSIDSWKNAGRVFKDSDKFDANDSILKDQTQEW
                     SGSATFTSDGKIRLFYTDFSGKHYGKQTLTTAQVNVSASDSSLNINGVEDYKSIFDGDG
                     KTYQNVQQFIDEGNYSSGDNHTLRDPHYVEDKGHKYLVFEANTGTEDGYQGEESLFNKA
                     YYGKSTSFFRQESQKLLQSDKKRTAELANGALGMIELNDDYTLKKVMKPLIASNTVTDE
                     IERANVFKMNGKWYLFTDSRGSKMTIDGITSNDIYMLGYVSNSLTGPYKPLNKTGLVLK
                     MDLDPNDVTFTYSHFAVPQAKGNNVVITSYMTNRGFYADKQSTFAPSFLLNIKGKKTSV
                     VKDSILEQGQLTVNK"
     gene            complement(473..1894)
                     /gene="sacB"
                     /label=sacB
     promoter        complement(1895..2340)
                     /gene="sacR"
                     /label=sacB promoter
                     /note="sacB promoter and control region"
     promoter        2488..2590
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     CDS             2591..3250
                     /codon_start=1
                     /gene="cm"
                     /product="chloramphenicol resistance protein"
                     /label=cm
                     /protein_id="AWK77090.1"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     gene            2591..3250
                     /gene="cm"
                     /label=cm
     rep_origin      4380..4968
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    5154..5294
                     /label=bom
                     /note="basis of mobility region from pBR322"