LOCUS       Exported                3685 bp ds-DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector pCI-YFP, complete sequence.
ACCESSION   JQ394803
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3685)
  AUTHORS   Anderson JC, Voigt CA, Arkin AP.
  TITLE     Environmental signal integration by a modular AND gate
  JOURNAL   Mol. Syst. Biol. 3, 133 (2007)
  PUBMED    17700541
REFERENCE   2  (bases 1 to 3685)
  AUTHORS   Salis HM, Mirsky EA, Voigt CA.
  TITLE     Automated design of synthetic ribosome binding sites to control 
            protein expression
  JOURNAL   Nat. Biotechnol. 27 (10), 946-950 (2009)
  PUBMED    19801975
REFERENCE   3  (bases 1 to 3685)
  AUTHORS   Tamsir A, Tabor JJ, Voigt CA.
  TITLE     Robust multicellular computing using genetically encoded NOR gates 
            and chemical wires
  JOURNAL   Nature 469 (7329), 212-215 (2011)
  PUBMED    21150903
REFERENCE   4  (bases 1 to 3685)
  AUTHORS   Moser F, Voigt CA.
  TITLE     Genetic Circuit Performance under Conditions Relevant for Industrial
            Bioreactors
  JOURNAL   Unpublished
REFERENCE   5  (bases 1 to 3685)
  AUTHORS   Anderson JC, Moser F, Tamsir A, Salis H.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-JAN-2012) Biological Engineering, MIT, 500 Tech 
            Square, Rm 209D, Cambridge, MA 02139, USA
REFERENCE   6  (bases 1 to 3685)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..3685
                     /organism="Cloning vector pCI-YFP"
                     /mol_type="other DNA"
                     /label=derived from Escherichia coli strain DH10b
                     /note="derived from Escherichia coli strain DH10b"
                     /db_xref="taxon:1175534"
     misc_feature    1..21
                     /label=biobrick suffix
                     /note="biobrick suffix"
     CDS             complement(239..1054)
                     /codon_start=1
                     /gene="KanR"
                     /product="KanR"
                     /label=KanR
                     /protein_id="AFI26427.1"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     gene            complement(239..1054)
                     /gene="KanR"
                     /label=KanR
     misc_feature    1386..2210
                     /label=p15A
                     /note="p15A"
     rep_origin      complement(1649..2193)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin."
     terminator      2683..2726
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    2729..2750
                     /label=biobrick prefix
                     /note="biobrick prefix"
     regulatory      2751..2799
                     /regulatory_class="promoter"
                     /note="PCI BBa_R0051"
     regulatory      2808..2819
                     /regulatory_class="ribosome_binding_site"
                     /note="BBa_B0034"
     RBS             2808..2819
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             2826..3545
                     /codon_start=1
                     /gene="YFP"
                     /product="YFP"
                     /label=YFP
                     /note="BBa_E0030"
                     /protein_id="AFI26426.1"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKLHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     gene            2826..3545
                     /gene="YFP"
                     /label=YFP
     regulatory      3557..3685
                     /regulatory_class="terminator"
                     /note="BBa_B0015"
     terminator      3565..3636
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      3652..3679
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"