LOCUS       Exported                4956 bp ds-DNA     circular SYN 17-DEC-2018
DEFINITION  BiFC vector pCHGC155, complete sequence.
ACCESSION   KC344838
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4956)
  AUTHORS   Gong Z, Lu H.
  TITLE     A new combination of fluorescent protein fragments suitable for 
            green-colored bimolecular fluorescence complementation (BiFC) assays
            in Schizosaccharomyces pombe
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4956)
  AUTHORS   Gong Z, Lu H.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2012) School of Life Science, Fudan University, 
            Room 618, Science Building, Handan Road 220, Shanghai 200433, China
REFERENCE   3  (bases 1 to 4956)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..4956
                     /organism="BiFC vector pCHGC155"
                     /mol_type="other DNA"
                     /db_xref="taxon:1306191"
     promoter        235..253
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     misc_feature    294..344
                     /label=linker
                     /note="linker"
     misc_feature    345..596
                     /note="GC155 (encoding amino acid residues 155-238 of 
                     mEGFP)"
     misc_feature    597..614
                     /label=6His tag
                     /note="6His tag"
     CDS             597..614
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     regulatory      624..1116
                     /regulatory_class="terminator"
                     /note="Saccharomyces cerevisiae LEU2 terminator"
     regulatory      1127..1505
                     /regulatory_class="promoter"
                     /note="Ashbya gossypii TEF1 promoter"
     gene            1162..2737
                     /label=hphMX6
                     /note="yeast selectable marker conferring hygromycin 
                     resistance"
     promoter        1162..1505
                     /label=TEF promoter
                     /note="Ashbya gossypii TEF promoter"
     CDS             1506..2534
                     /codon_start=1
                     /product="hph"
                     /label=hph
                     /note="hygromycin B resistance gene"
                     /protein_id="AGH62558.1"
                     /translation="MGKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGR
                     GYVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDL
                     PETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHW
                     QTVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAM
                     FGDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVD
                     GNFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRA
                     KE"
     terminator      2540..2737
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
     regulatory      2541..2772
                     /regulatory_class="terminator"
                     /note="Ashbya gossypii TEF1 terminator"
     promoter        complement(2845..2863)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      3106..3725
                     /label=pBR322 origin
                     /note="pBR322 origin"
     rep_origin      complement(3121..3709)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3880..4740)
                     /codon_start=1
                     /product="AmpR"
                     /label=AmpR
                     /note="beta-lactamase"
                     /protein_id="AGH62559.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(4741..4845)
                     /gene="bla"
                     /label=AmpR promoter