LOCUS       Exported                6398 bp ds-DNA     circular SYN 17-DEC-2018
DEFINITION  Shuttle vector pCGL0609, complete sequence.
ACCESSION   AF092931
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6398)
  AUTHORS   Reyes O, Guyonvarch A, Bonamy C, Salti V, David F, Leblon G.
  TITLE     Integron-bearing vectors: a method suitable for stable chromosomal
            integration in highly restrictive corynebacteria
  JOURNAL   Gene 107 (1), 61-68 (1991)
  PUBMED    1660430
REFERENCE   2  (bases 1 to 6398)
  AUTHORS   Ankri S, Reyes O, Leblon G.
  TITLE     Electrotransformation of highly DNA-restrictive corynebacteria with 
            synthetic DNA
  JOURNAL   Plasmid 35 (1), 62-66 (1996)
  PUBMED    8693028
REFERENCE   3  (bases 1 to 6398)
  AUTHORS   Ben-Samoun K, Leblon G, Reyes O.
  TITLE     Positively regulated expression of the Escherichia coli araBAD 
            promoter in Corynebacterium glutamicum
  JOURNAL   FEMS Microbiol. Lett. 174 (1), 125-130 (1999)
  PUBMED    10234830
REFERENCE   4  (bases 1 to 6398)
  AUTHORS   Ben-Samoun K, Leblon G, Reyes O.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-SEP-1998) Laboratoire de Biologie Moleculaire des 
            Corynebacteries, Institut de Genetique et Microbiologie URA 2225, 
            Universite de Paris XI, Orsay F-91405, France
REFERENCE   5  (bases 1 to 6398)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..6398
                     /organism="Shuttle vector pCGL0609"
                     /lab_host="Corynebacterium glutamicum"
                     /mol_type="genomic DNA"
                     /note="Corynebacterium glutamicum/Escherichia coli shuttle 
                     vector; derived from pCGL0243 (GenBank accession AF091268),
                     pBl1 (GenBank accession AF092037) and pJH1 (GenBank 
                     accession V01547)"
                     /db_xref="taxon:86130"
     misc_feature    1..140
                     /note="multiple cloning site of plasmid pCGL0243 in GenBank
                     Accession Number AF091268"
     primer_bind     72..88
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    141..1629
                     /note="derived from plasmid pJH1 in GenBank Accession 
                     Number V01547"
     CDS             675..1469
                     /codon_start=1
                     /gene="aph3"
                     /product="aminoglycoside phosphotransferase"
                     /label=aph3
                     /protein_id="AAD08691.1"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     gene            675..1469
                     /gene="aph3"
                     /label=aph3
     misc_feature    1630..6398
                     /note="replicative region of plasmid pCGL0243 in GenBank 
                     Accession Number AF091268"
     gene            complement(2411..3799)
                     /gene="repBl1"
                     /label=repBl1
     CDS             complement(2495..3700)
                     /codon_start=1
                     /gene="repBl1"
                     /product="replicase"
                     /label=repBl1
                     /note="minimally known pBl1 region able to promote 
                     replication"
                     /protein_id="AAD08692.1"
                     /translation="MYKITNSKALAGCHRWRRDEAVAVSWSSNGASQFEGLQNSHSRWG
                     SPLAELEVMGERRIELAIATKNHLAAGGALMMFVGTVRHNRSQSFAQVEAGIKTAYSSM
                     VKTSQWKKERARYGVEHTYSDYEVTDSWANGWHLHRNMLLFLDRPLSDDELKAFEDSMF
                     SRWSAGVVKAGMDAPLREHGVKLDQVSTWGGDAAKMATYLAKGMSQELTGSATKTASKG
                     SYTPFQMLDMLADQSDAGEDMDAVLVARWREYEVGSKNLRSSWSRGAKRALGIDYIDAD
                     VRREMEEELYKLAGLEAPERVESTRVAVALVKPDDWKLIQSDFAVRQYVLDCVDKAKDV
                     AAAQRVANEVLASLGVDSTPCMIVMDDVDLDAVLPTHGDATKRDLNAAVFAGNEQTILR
                     TH"
     rep_origin      5889..6317
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"