LOCUS       Exported                6241 bp ds-DNA     circular SYN 17-DEC-2018
DEFINITION  Shuttle vector pCGL0482, complete sequence.
ACCESSION   AF092036
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6241)
  AUTHORS   Reyes O, Guyonvarch A, Bonamy C, Salti V, David F, Leblon G.
  TITLE     Integron-bearing vectors: a method suitable for stable chromosomal
            integration in highly restrictive corynebacteria
  JOURNAL   Gene 107 (1), 61-68 (1991)
  PUBMED    1660430
REFERENCE   2  (bases 1 to 6241)
  AUTHORS   Ankri S, Reyes O, Leblon G.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-1998) Laboratoire de Biologie Moleculaire des 
            Corynebacteries, Institut de Genetique et Microbiologie URA 2225, 
            Universite de Paris XI, Orsay F-91405 Orsay cedex, France
REFERENCE   3  (bases 1 to 6241)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..6241
                     /organism="Shuttle vector pCGL0482"
                     /lab_host="Corynebacterium glutamicum"
                     /mol_type="other DNA"
                     /note="Corynebacterium glutamicum-Escherichia coli shuttle 
                     vector; derived from pACYC184 (GenBank accession X06403), 
                     pBl1 (GenBank accession AF092037), and pCGL0243 (GenBank 
                     accession AF091268)"
                     /db_xref="taxon:86251"
     misc_feature    1..141
                     /note="multiple cloning site of plasmid pCGL0243 in GenBank
                     Accession Number AF091268"
     primer_bind     72..88
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    145..1470
                     /note="derived from plasmid pACYC184 in GenBank Accession 
                     Number X06403"
     CDS             complement(447..1106)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=cat
                     /protein_id="AAD08688.1"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     gene            complement(447..1106)
                     /gene="cat"
                     /label=cat
     promoter        complement(1107..1209)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     misc_feature    1471..6241
                     /note="replicative region of plasmid pCGL0243 in GenBank 
                     Accession Number AF091268"
     CDS             complement(2338..3543)
                     /codon_start=1
                     /gene="repBl1"
                     /product="replicase"
                     /label=repBl1
                     /note="minimally known pBl1 region able to promote 
                     replication"
                     /protein_id="AAD08689.1"
                     /translation="MYKITNSKALAGCHRWRRDEAVAVSWSSNGASQFEGLQNSHSRWG
                     SPLAELEVMGERRIELAIATKNHLAAGGALMMFVGTVRHNRSQSFAQVEAGIKTAYSSM
                     VKTSQWKKERARYGVEHTYSDYEVTDSWANGWHLHRNMLLFLDRPLSDDELKAFEDSMF
                     SRWSAGVVKAGMDAPLREHGVKLDQVSTWGGDAAKMATYLAKGMSQELTGSATKTASKG
                     SYTPFQMLDMLADQSDAGEDMDAVLVARWREYEVGSKNLRSSWSRGAKRALGIDYIDAD
                     VRREMEEELYKLAGLEAPERVESTRVAVALVKPDDWKLIQSDFAVRQYVLDCVDKAKDV
                     AAAQRVANEVLASLGVDSTPCMIVMDDVDLDAVLPTHGDATKRDLNAAVFAGNEQTILR
                     TH"
     gene            complement(2338..3543)
                     /gene="repBl1"
                     /label=repBl1
     rep_origin      5732..6160
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"