LOCUS       Exported                3147 bp ds-DNA     circular SYN 14-DEC-2018
DEFINITION  Integration vector pCGL0289, complete sequence.
ACCESSION   AF092035
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3147)
  AUTHORS   Reyes O, Guyonvarch A, Bonamy C, Salti V, David F, Leblon G.
  TITLE     Integron-bearing vectors: a method suitable for stable chromosomal
            integration in highly restrictive corynebacteria
  JOURNAL   Gene 107 (1), 61-68 (1991)
  PUBMED    1660430
REFERENCE   2  (bases 1 to 3147)
  AUTHORS   Ankri S, Reyes O, Leblon G.
  TITLE     Electrotransformation of highly DNA-restrictive corynebacteria with 
            synthetic DNA
  JOURNAL   Plasmid 35 (1), 62-66 (1996)
  PUBMED    8693028
REFERENCE   3  (bases 1 to 3147)
  AUTHORS   Ankri S, Reyes O, Leblon G.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-1998) Laboratoire de Biologie Moleculaire des 
            Corynebacteries, Institut de Genetique et Microbiologie URA 2225, 
            Universite de Paris XI, Orsay F-91405 Orsay cedex, France
REFERENCE   4  (bases 1 to 3147)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..3147
                     /organism="Integration vector pCGL0289"
                     /lab_host="Corynebacterium glutamicum"
                     /mol_type="other DNA"
                     /note="derived from pJHI (GenBank accession V01547), 
                     pACYC184 (GenBank accession X06403) and pBl1 (ATCC 
                     accession 21086)"
                     /db_xref="taxon:86129"
     misc_feature    1..1335
                     /note="derived from plasmid pJH1 in GenBank Accession 
                     Number V01547"
     CDS             complement(4..798)
                     /codon_start=1
                     /gene="aph3"
                     /product="aminoglycoside phosphotransferase"
                     /label=aph3
                     /note="kanamycin resistance determinant"
                     /protein_id="AAD08686.1"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     gene            complement(4..798)
                     /gene="aph3"
                     /label=aph3
     misc_feature    1301..1754
                     /note="chloramphenicol sensitive cat gene fragment derived 
                     from plasmid pACYC184 in GenBank Accession Number X06403"
     misc_feature    1336..1458
                     /label=multiple cloning site
                     /note="multiple cloning site"
     misc_feature    1459..3147
                     /note="minimal plasmid pB11 (GenBank Accession Number 
                     AF092037) fragment able to promote autonomous replication 
                     in Corynebacterium glutamicum"
     CDS             complement(1843..3048)
                     /codon_start=1
                     /gene="repBl1"
                     /product="replicase"
                     /label=repBl1
                     /protein_id="AAD08687.1"
                     /translation="MYKITNSKALAGCHRWRRDEAVAVSWSSNGASQFEGLQNSHSRWG
                     SPLAELEVMGERRIELAIATKNHLAAGGALMMFVGTVRHNRSQSFAQVEAGIKTAYSSM
                     VKTSQWKKERARYGVEHTYSDYEVTDSWANGWHLHRNMLLFLDRPLSDDELKAFEDSMF
                     SRWSAGVVKAGMDAPLREHGVKLDQVSTWGGDAAKMATYLAKGMSQELTGSATKTASKG
                     SYTPFQMLDMLADQSDAGEDMDAVLVARWREYEVGSKNLRSSWSRGAKRALGIDYIDAD
                     VRREMEEELYKLAGLEAPERVESTRVAVALVKPDDWKLIQSDFAVRQYVLDCVDKAKDV
                     AAAQRVANEVLASLGVDSTPCMIVMDDVDLDAVLPTHGDATKRDLNAAVFAGNEQTILR
                     TH"
     gene            complement(1843..3048)
                     /gene="repBl1"
                     /label=repBl1