LOCUS       Exported                7228 bp ds-DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector pCgeA-C-thr, complete sequence.
ACCESSION   KF933409
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7228)
  AUTHORS   Iwanicki A, Hinc K, Stasilojc M, Piatek I, Grela A, Lega T, 
            Obuchowski M.
  TITLE     Vector system for spore sufrace display
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7228)
  AUTHORS   Iwanicki A, Hinc K, Stasilojc M, Piatek I, Grela A, Lega T, 
            Obuchowski M.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2013) Laboratory of Molecular Bacteriology, 
            Medical University of Gdansk, Debinki 1, Gdansk 80-211, Poland
REFERENCE   3  (bases 1 to 7228)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Assembly Method       :: Vector NTI Advance v. 10
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7228
                     /organism="Cloning vector pCgeA-C-thr"
                     /mol_type="other DNA"
                     /db_xref="taxon:1449687"
     CDS             247..879
                     /codon_start=1
                     /gene="thrC"
                     /product="ThrC"
                     /label=thrC
                     /note="N terminal part of thrC"
                     /protein_id="AHG54639.1"
                     /translation="MWKGLIHQYKEFLPVTDQTPALTLHEGNTPLIHLPKLSEQLGIEL
                     HVKTEGVNPTGSFKDRGMVMAVAKAKEEGNDTIMCASTGNTSAAAAAYAARANMKCIVI
                     IPNGKIAFGKLAQAVMYGAEIIAIDGNFDDALKIVRSICEKSPIALVNSVNPYRIEGQK
                     TAAFEIVHNGFPYAKYGQTWPARLLLFLTPDQLVMVATGAQLELRRY"
     gene            247..879
                     /gene="thrC"
                     /label=thrC
     misc_feature    complement(1124..1149)
                     /label=multiple cloning site
                     /note="multiple cloning site"
     gene            complement(1135..1612)
                     /gene="cgeA"
                     /label=cgeA
     CDS             complement(1135..1548)
                     /codon_start=1
                     /gene="cgeA"
                     /product="CgeA"
                     /label=cgeA
                     /note="spore coat protein"
                     /protein_id="AHG54636.1"
                     /translation="MSSENAQLKKDLIKAVLSPLFPTATEGGENMDSNLKALLDAAIDQ
                     KVDESETVTAESILDPSLPARWIFARITPGTTISIVTDSGDMIGPVVFVAFDQVHGIVF
                     VTQESSVTPAGQATTLIDVDKVESVTFFSGSGR"
     regulatory      complement(1557..1562)
                     /regulatory_class="ribosome_binding_site"
                     /gene="cgeA"
     regulatory      complement(1584..1592)
                     /regulatory_class="minus_10_signal"
                     /gene="cgeA"
     regulatory      complement(1609..1612)
                     /regulatory_class="minus_35_signal"
                     /gene="cgeA"
     CDS             2109..2861
                     /codon_start=1
                     /gene="trpC"
                     /product="TrpC"
                     /label=trpC
                     /protein_id="AHG54640.1"
                     /translation="MLEKIIKQKKEEVKTLVLPVEQPFEKRSFKEAPASPNRFIGLIAE
                     VKKASPSKGLIKEDFVPVQIAKDYEAAKADAISVLTDTPFFQGENSYLSDVKRAVSIPV
                     LRKDFIIDSLQVEESRRIGADAILLIGEVLDPLHLHELYLEAGEKGMDVLVEVHDASTL
                     EQILKVFTPDILGVNNRNLKTFETSVKQTEQIASLVPKESLLVSESGIGSLEHLTFVNE
                     HGARAVLIGESLMRQTSQRKAIHALFRE"
     gene            2109..2861
                     /gene="trpC"
                     /label=trpC
     CDS             3291..3719
                     /codon_start=1
                     /gene="thrC"
                     /product="ThrC"
                     /label=thrC
                     /note="C-terminal part of thrC"
                     /protein_id="AHG54638.1"
                     /translation="MRGFEAEGAAAIVRNEVIENPETIATAIRIGNPASWDKAVKAAEE
                     SNGKIDEVTDDEILHAYQLIARVEGVFAEPGSCASIAGVLKQVKSGEIPKGSKVVAVLT
                     GNGLKDPNTAVDISEIKPVTLPTDEDSILEYVKGAARV"
     gene            3291..3719
                     /gene="thrC"
                     /label=thrC
     CDS             3716..4126
                     /codon_start=1
                     /gene="thrB"
                     /product="ThrB"
                     /label=thrB
                     /note="N-terminal part of thrB"
                     /protein_id="AHG54637.1"
                     /translation="MNEADMLFSVTVPGSTANLGPGFDSVGMALSRYLKLTVFESDKWS
                     FEAETETVAGINSSSFNFLTSSCIGTISRSSNPSSFTSIRFNQHSKNLTGHRQFFEQCA
                     NYQLFLQFIQGTGEPACNPSCSIMRNDPHPVS"
     gene            3716..4126
                     /gene="thrB"
                     /label=thrB
     rep_origin      complement(5391..6063)
                     /direction=LEFT
                     /label=ColE1
                     /note="ColE1"
     rep_origin      complement(5399..5987)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(6158..7018)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=bla
                     /protein_id="AHG54635.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     gene            complement(6158..7018)
                     /gene="bla"
                     /label=bla
     promoter        complement(7019..7123)
                     /gene="bla"
                     /label=AmpR promoter