LOCUS       Exported                9113 bp ds-DNA     circular SYN 17-DEC-2018
DEFINITION  Promoter-probe vector pCG1408, complete sequence.
ACCESSION   U21228
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9113)
  AUTHORS   David M, Bartz SL, Weaver JD, Kelley JL.
  TITLE     Isolation and Characterization of Promoter Sequences from 
            Clavibacter xyli subsp. cynodontis
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9113)
  AUTHORS   Taylor J, Stearman RS, Uratani BB.
  TITLE     Development of a native plasmid as a cloning vector in Clavibacter 
            xyli subsp. cynodontis
  JOURNAL   Plasmid 29 (3), 241-244 (1993)
  PUBMED    7689234
REFERENCE   3  (bases 1 to 9113)
  AUTHORS   David M.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-FEB-1995) Michael David, Crop Genetics, 10150 Old 
            Columbia Rd, Columbia, MD 21046, USA, 21046
REFERENCE   4  (bases 1 to 9113)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..9113
                     /organism="Cloning vector pCG1408"
                     /focus
                     /mol_type="genomic DNA"
                     /db_xref="taxon:41458"
     source          8414..5464
                     /organism="Cloning vector pKK232-8"
                     /mol_type="genomic DNA"
                     /note="region derived from vector pKK232-8, GenBank 
                     Accession Number U13859"
                     /db_xref="taxon:74582"
     CDS             88..747
                     /codon_start=1
                     /product="chloramphenicol acetyltransferase"
                     /label=chloramphenicol acetyltransferase
                     /protein_id="AAA70410.1"
                     /translation="MEKKITGYTTVDISQSHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     terminator      1182..1268
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     regulatory      1185..1228
                     /regulatory_class="terminator"
                     /note="T1"
     regulatory      1360..1388
                     /regulatory_class="terminator"
                     /note="T2"
     terminator      1360..1387
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      1680..1766
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     regulatory      1684..1726
                     /regulatory_class="terminator"
                     /note="T1"
     regulatory      1858..1885
                     /regulatory_class="terminator"
                     /note="T2"
     terminator      1858..1885
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     promoter        2597..2701
                     /gene="bla"
                     /label=AmpR promoter
     CDS             2702..3562
                     /codon_start=1
                     /product="beta-lactamase"
                     /label=beta-lactamase
                     /protein_id="AAA70411.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      3733..4321
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     rep_origin      4084..4508
                     /label=E. coli origin of replication
                     /note="E. coli origin of replication"
     misc_feature    4507..4647
                     /label=bom
                     /note="basis of mobility region from pBR322"
     CDS             complement(4749..4940)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     rep_origin      5465..8413
                     /label=Clavibacter origin of replication
                     /note="Clavibacter origin of replication"
                     /citation="[1]"
                     /citation="[2]"
     promoter        complement(8725..8829)
                     /gene="bla"
                     /label=AmpR promoter
     terminator      8944..9030
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     regulatory      8947..8990
                     /regulatory_class="terminator"
                     /note="T1"
     misc_feature    9109..26
                     /label=polylinker
                     /note="polylinker"