LOCUS       Exported                6448 bp ds-DNA     circular SYN 17-DEC-2018
DEFINITION  Mammalian vector pCFP-N-GW, complete sequence.
ACCESSION   LT726798
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6448)
  AUTHORS   De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman 
            Fonseca M, Vanhoucke M, Beyaert R.
  TITLE     BCCM/LMBP Plasmid collection
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6448)
  AUTHORS   De Schamphelaire W.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2017) BCCM/LMBP, Universiteit Gent, 
            Technologiepark 927, 9052, BELGIUM
REFERENCE   3  (bases 1 to 6448)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..6448
                     /organism="Mammalian vector pCFP-N-GW"
                     /mol_type="other DNA"
                     /note="BCCM/LMBP Plasmid collection (Ghent 
                     University,Belgium) accession number LMBP 8366."
                     /db_xref="taxon:1945164"
     rep_origin      39..365
                     /label=pMB1 ori
                     /note="pMB1 ori"
     regulatory      372..939
                     /regulatory_class="promoter"
                     /note="hCMV-IE promoter and enhancer"
     enhancer        432..735
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        736..939
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     regulatory      978..987
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     CDS             984..1700
                     /codon_start=1
                     /note="unnamed protein product; ECFP"
                     /protein_id="SJL86514.1"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTWGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISHNVYITADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     regulatory      complement(1719..1843)
                     /regulatory_class="attenuator"
                     /note="attR1"
     protein_bind    1719..1843
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     regulatory      1851..1903
                     /regulatory_class="promoter"
                     /note="lacUV5 promoter"
     promoter        1868..1898
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             1952..2611
                     /codon_start=1
                     /note="unnamed protein product; chloramphenicol resistance"
                     /protein_id="SJL86515.1"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     CDS             2953..3258
                     /codon_start=1
                     /note="unnamed protein product; ccdB"
                     /protein_id="SJL86516.1"
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     regulatory      3299..3423
                     /regulatory_class="attenuator"
                     /note="attR2"
     protein_bind    complement(3299..3423)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     misc_feature    3431..3483
                     /label=MCS
                     /note="multiple cloning site"
     regulatory      complement(3585..3681)
                     /regulatory_class="terminator"
                     /note="SV40 polyA late"
     regulatory      3585..3681
                     /regulatory_class="terminator"
                     /note="SV40 polyA early"
     polyA_signal    3607..3728
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(3735..4190)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 ori"
     regulatory      4191..4286
                     /regulatory_class="promoter"
                     /note="ampicillin resistance promoter"
     promoter        4217..4321
                     /gene="bla"
                     /label=AmpR promoter
     misc_feature    4287..4321
                     /label=5 UTR of amp, incl. RBS
                     /note="5 UTR of amp, incl. RBS"
     promoter        4323..4680
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     regulatory      4342..4623
                     /regulatory_class="promoter"
                     /note="SV40 early promoter"
     rep_origin      4531..4666
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     rep_origin      4580..4655
                     /label=SV40 ori
                     /note="SV40 ori"
     CDS             4715..5509
                     /codon_start=1
                     /note="unnamed protein product; Tn5 neomycin resistance"
                     /protein_id="SJL86517.1"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     regulatory      5700..5769
                     /regulatory_class="terminator"
                     /note="HSV-TK polyA"
     polyA_signal    5741..5788
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      6117..257
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"