LOCUS       Exported                5291 bp ds-DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector pCFB, complete sequence.
ACCESSION   AF311737
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5291)
  AUTHORS   Felts K, Vaillancourt P, Grafsky AJ.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-OCT-2000) Technical Services, Stratagene, 11011 N. 
            Torrey Pines Rd., La Jolla, CA 92037, USA
REFERENCE   2  (bases 1 to 5291)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..5291
                     /organism="Cloning vector pCFB"
                     /lab_host="Escherichia coli"
                     /mol_type="genomic DNA"
                     /note="synthetic retroviral plasmid vector; used with 
                     Stratagenes ViraPort system"
                     /db_xref="taxon:141214"
     repeat_region   209..935
                     /label=5
                     /note="5"
     regulatory      249..779
                     /regulatory_class="promoter"
                     /note="CMV"
     enhancer        268..571
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        572..775
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     regulatory      761..767
                     /regulatory_class="TATA_box"
     misc_feature    791
                     /label=transcription initiation start site
                     /note="transcription initiation start site"
     misc_feature    935..2221
                     /label=Psi+
                     /note="Psi+"
     regulatory      993..997
                     /regulatory_class="ribosome_binding_site"
                     /note="SD"
     misc_feature    998..1355
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     misc_feature    1411..1928
                     /label=gag packaging signal sequence
                     /note="gag packaging signal sequence"
     CDS             1420..1836
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     misc_feature    1846..2219
                     /label=pol region
                     /note="Moloney murine leukemia virus (MMLV) pol region 
                     containing the splice acceptor site"
     regulatory      1926..1928
                     /regulatory_class="terminator"
                     /note="SA"
     misc_feature    2232..2254
                     /label=multiple cloning site
                     /note="multiple cloning site"
     repeat_region   2338..2931
                     /label=3
                     /note="3"
     LTR             2338..2931
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     rep_origin      3044
                     /label=ColE1 replication origin
                     /note="ColE1 replication origin"
     misc_feature    3142..3282
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(3467..4055)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(4226..5086)
                     /codon_start=1
                     /product="beta lactamase"
                     /label=beta lactamase
                     /note="ampicillin resistance"
                     /protein_id="AAG30933.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(5087..5191)
                     /gene="bla"
                     /label=AmpR promoter