LOCUS       Exported                8353 bp ds-DNA     circular SYN 17-DEC-2018
DEFINITION  Chloroplast expression vector pCEV2, complete sequence.
ACCESSION   KY930498
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8353)
  AUTHORS   Chen P-Y., To K-Y.
  TITLE     Construction of chloroplast expression vectors for higher plants
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8353)
  AUTHORS   Chen P-Y., To K-Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-APR-2017) Agricultural Biotechnology Research Center, 
            Academia Sinica, No. 128, Academia Road, Section 2, Nankang, Taipei 
            11529, Taiwan
REFERENCE   3  (bases 1 to 8353)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..8353
                     /organism="Chloroplast expression vector pCEV2"
                     /mol_type="other DNA"
                     /db_xref="taxon:2127038"
     primer_bind     complement(26..42)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    50..66
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(74..104)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    119..140
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(428..1016)
                     /direction=LEFT
                     /label=pUC19 replication origin
                     /note="pUC19 replication origin"
     CDS             complement(1187..2047)
                     /codon_start=1
                     /product="beta-lactamase"
                     /label=beta-lactamase
                     /note="confers ampicillin resistance"
                     /protein_id="AVQ67765.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(2048..2152)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     2626..2642
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    2655..4648
                     /note="tomato left targeting region; contains 16S rRNA and 
                     trnI"
     rep_origin      4072..4153
                     /note="chloroplast replication origin; oriA"
     regulatory      4655..4902
                     /regulatory_class="promoter"
                     /note="atpI promoter; PatpI"
     misc_feature    4903..4918
                     /label=cloning site
                     /note="cloning site"
     regulatory      4919..5087
                     /regulatory_class="terminator"
                     /note="rps16 terminator; Trps16"
     regulatory      5094..5312
                     /regulatory_class="promoter"
                     /note="rrn promoter; Prrn"
     CDS             5319..6110
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside 3-adenyltransferase"
                     /label=aadA
                     /note="confers spectinomycin resistance"
                     /protein_id="AVQ67766.1"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     gene            5319..6110
                     /gene="aadA"
                     /label=aadA
     regulatory      6117..6338
                     /regulatory_class="terminator"
                     /note="psbA terminator; TpsbA"
     misc_feature    6345..8348
                     /note="tomato right targeting region; contains trnA and 23S
                     rRNA"