LOCUS       Exported                6860 bp ds-DNA     circular SYN 17-DEC-2018
DEFINITION  Expression vector pCeMM-NTAP(GS), complete sequence.
ACCESSION   EF467047
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6860)
  AUTHORS   Burckstummer T, Bennett KL, Preradovic A, Schutze G, Hantschel O, 
            Superti-Furga G, Bauch A.
  TITLE     An efficient tandem affinity purification procedure for interaction 
            proteomics in mammalian cells
  JOURNAL   Nat. Methods 3 (12), 1013-1019 (2006)
  PUBMED    17060908
REFERENCE   2  (bases 1 to 6860)
  AUTHORS   Burckstummer T, Bennett KL, Preradovic A, Schutze G, Hantschel O, 
            Superti-Furga G, Bauch A.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-MAR-2007) Directors Laboratory, Research Center for 
            Molecular Medicine, Lazarettgasse 19, Vienna 1090, Austria
REFERENCE   3  (bases 1 to 6860)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..6860
                     /organism="Expression vector pCeMM-NTAP(GS)"
                     /mol_type="other DNA"
                     /db_xref="taxon:433597"
     CDS             380..400
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             407..520
                     /codon_start=1
                     /product="streptavidin-binding peptide"
                     /label=SBP
                     /note="selected from a peptide library; binds streptavidin 
                     with nanomolar affinity (Keefe et al., 2001)"
                     /translation="MDEKTTGWRGGHVVEGLAGELEQLRARLEHHPQGQREP"
     misc_feature    604..1191
                     /label=IRES
                     /note="IRES"
     misc_feature    607..1192
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     CDS             1193..1912
                     /codon_start=1
                     /gene="GFP"
                     /product="GFP"
                     /label=GFP
                     /protein_id="ABO76908.1"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITHGMDELYK"
     gene            1193..1912
                     /gene="GFP"
                     /label=GFP
     repeat_region   2078..2671
                     /label=3 LTR
                     /note="3 LTR"
     LTR             2200..2669
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     CDS             2754..3614
                     /codon_start=1
                     /product="ampicillin resistance protein"
                     /label=ampicillin resistance protein
                     /note="APr"
                     /protein_id="ABO76909.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      3617..4537
                     /label=ColE1 Ori
                     /note="ColE1 Ori"
     rep_origin      3785..4373
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    4661..4682
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        4697..4727
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4735..4751
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4759..4775
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     regulatory      4792..5368
                     /regulatory_class="promoter"
                     /note="hCMV"
     enhancer        4857..5160
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        5165..5368
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     repeat_region   5369..5554
                     /label=5 LTR
                     /note="5 LTR"
     misc_feature    5607..5964
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             6029..6445
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     promoter        6792..6810
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             6853..520
                     /codon_start=1
                     /gene="NTAP(GS)"
                     /product="NTAP(GS)"
                     /label=NTAP(GS)
                     /protein_id="ABO76907.1"
                     /translation="MEFMGTPAVTTYKLVINGKTLKGETTTKAVDAETAEKAFKQYAND
                     NGVDGVWTYDDATKTFTVTEVNTPAVTTYKLVINGKTLKGETTTKAVDAETAEKAFKQY
                     ANDNGVDGVWTYDDATKTFTVTEIDENLYFQGGSMDEKTTGWRGGHVVEGLAGELEQLR
                     ARLEHHPQGQREP"
     gene            6853..520
                     /gene="NTAP(GS)"
                     /label=NTAP(GS)