LOCUS       Exported                6920 bp ds-DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector pCE70, complete sequence.
ACCESSION   DQ096479
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6920)
  AUTHORS   Merighi M, Ellermeier CD, Slauch JM, Gunn JS.
  TITLE     Resolvase-IVET Analysis of the Salmonella enterica sv. typhimurium 
            PhoP and PmrA regulons in BALB/c mice
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6920)
  AUTHORS   Smith JN, Ellermeier CD, Dyszel J, Altier C, Young GM, Lawhon SD, 
            Adams LG, Konjufca VK, Curtiss R III, Slauch JM, Ahmer BMM.
  TITLE     Recording of SdiA activity during transit of Salmonella through 
            animals
  JOURNAL   Unpublished
REFERENCE   3  (bases 1 to 6920)
  AUTHORS   Ellermeier CD, Slauch JM.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2005) Microbiology, University of Illinois 
            Urbana-Champaign, 601 S. Goodwin Ave. B103 CLSL, Urbana, IL 61801, 
            USA
REFERENCE   4  (bases 1 to 6920)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..6920
                     /organism="Cloning vector pCE70"
                     /mol_type="other DNA"
                     /db_xref="taxon:332945"
     misc_feature    40..74
                     /note="FRT; Flp recombinase target site"
     protein_bind    complement(40..73)
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     gene            82..650
                     /gene="tnpR"
                     /label=tnpR
     regulatory      82..89
                     /regulatory_class="ribosome_binding_site"
                     /gene="tnpR"
     CDS             99..650
                     /codon_start=1
                     /gene="tnpR"
                     /product="TnpR"
                     /label=tnpR
                     /note="resolvase; derived from Tn1000"
                     /protein_id="AAZ05410.1"
                     /translation="MPLFGYARVSTSQQSLDIQVRALKDAGVKANRIFTDKASGSSSDP
                     KGLDLLRMKVEEGDVILVKKLDRLGRDTADMIQLIKEFDAQGVSIRFIDDGISTDGEMG
                     KMVVTILSAVAQAERQRILERTNEGRQEAMAKGVVFGRKRKIDRDAVLNMWQQGLGASH
                     ISKTMNIPRSTVYKVINESN"
     CDS             675..3749
                     /codon_start=1
                     /gene="lacZ"
                     /product="beta-galactosidase"
                     /label=lacZ
                     /protein_id="AAZ05411.1"
                     /translation="MTMITDSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEART
                     DRPSQQLRSLNGEWRFAWFPAPEAVPESWLECDLPEADTVVVPSNWQMHGYDAPIYTNV
                     TYPITVNPPFVPTENPTGCYSLTFNVDESWLQEGQTRIIFDGVNSAFHLWCNGRWVGYG
                     QDSRLPSEFDLSAFLRAGENRLAVMVLRWSDGSYLEDQDMWRMSGIFRDVSLLHKPTTQ
                     ISDFHVATRFNDDFSRAVLEAEVQMCGELRDYLRVTVSLWQGETQVASGTAPFGGEIID
                     ERGGYADRVTLRLNVENPKLWSAEIPNLYRAVVELHTADGTLIEAEACDVGFREVRIEN
                     GLLLLNGKPLLIRGVNRHEHHPLHGQVMDEQTMVQDILLMKQNNFNAVRCSHYPNHPLW
                     YTLCDRYGLYVVDEANIETHGMVPMNRLTDDPRWLPAMSERVTRMVQRDRNHPSVIIWS
                     LGNESGHGANHDALYRWIKSVDPSRPVQYEGGGADTTATDIICPMYARVDEDQPFPAVP
                     KWSIKKWLSLPGETRPLILCEYAHAMGNSLGGFAKYWQAFRQYPRLQGGFVWDWVDQSL
                     IKYDENGNPWSAYGGDFGDTPNDRQFCMNGLVFADRTPHPALTEAKHQQQFFQFRLSGQ
                     TIEVTSEYLFRHSDNELLHWMVALDGKPLASGEVPLDVAPQGKQLIELPELPQPESAGQ
                     LWLTVRVVQPNATAWSEAGHISAWQQWRLAENLSVTLPAASHAIPHLTTSEMDFCIELG
                     NKRWQFNRQSGFLSQMWIGDKKQLLTPLRDQFTRAPLDNDIGVSEATRIDPNAWVERWK
                     AAGHYQAEAALLQCTADTLADAVLITTAHAWQHQGKTLFISRKTYRIDGSGQMAITVDV
                     EVASDTPHPARIGLNCQLAQVAERVNWLGLGPQENYPDRLTAACFDRWDLPLSDMYTPY
                     VFPSENGLRCGTRELNYGPHQWRGDFQFNISRYSQQQLMETSHRHLLHAEEGTWLNIDG
                     FHMGIGGDDSWSPSVSAEFQLSAGRYHYQLVWCQK"
     gene            675..3749
                     /gene="lacZ"
                     /label=lacZ
     primer_bind     complement(695..711)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             3801..5054
                     /codon_start=1
                     /gene="lacY"
                     /product="lactose permease"
                     /label=lacY
                     /note="LacY"
                     /protein_id="AAZ05412.1"
                     /translation="MYYLKNTNFWMFGLFFFFYFFIMGAYFPFFPIWLHDINHISKSDT
                     GIIFAAISLFSLLFQPLFGLLSDKLGLRKYLLWIITGMLVMFAPFFIFIFGPLLQYNIL
                     VGSIVGGIYLGFCFNAGAPAVEAFIEKVSRRSNFEFGRARMFGCVGWALCASIVGIMFT
                     INNQFVFWLGSGCALILAVLLFFAKTDAPSSATVANAVGANHSAFSLKLALELFRQPKL
                     WFLSLYVIGVSCTYDVFDQQFANFFTSFFATGEQGTRVFGYVTTMGELLNASIMFFAPL
                     IINRIGGKNALLLAGTIMSVRIIGSSFATSALEVVILKTLHMFEVPFLLVGCFKYITSQ
                     FEVRFSATIYLVCFCFFKQLAMIFMSVLAGNMYESIGFQGAYLVLGLVALGFTLISVFT
                     LSGPGPLSLLRRQVNEVA"
     gene            3801..5054
                     /gene="lacY"
                     /label=lacY
     regulatory      5260..5289
                     /regulatory_class="terminator"
                     /note="histidine bi-directional rho-independent terminator 
                     from Salmonella typhimurium"
     CDS             complement(5537..6352)
                     /codon_start=1
                     /gene="aph"
                     /product="kanamycin resistance"
                     /label=aph
                     /protein_id="AAZ05413.1"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     gene            complement(5537..6352)
                     /gene="aph"
                     /label=aph
     rep_origin      6476..6911
                     /label=oriR6K pi-dependent origin of replication
                     /note="oriR6K pi-dependent origin of replication"
     rep_origin      6522..6910
                     /label=R6K gamma ori
                     /note="gamma replication origin from E. coli plasmid R6K; 
                     requires the R6K initiator protein pi for replication"