LOCUS       Exported                3919 bp ds-DNA     circular SYN 17-DEC-2018
DEFINITION  Expression vector pCDS-At, complete sequence.
ACCESSION   GU828015
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3919)
  AUTHORS   Kaiser J, Rohdich F.
  TITLE     Thiazolopyrimidine inhibitors of 2-methylerythritol 
            2,4-cyclodiphosphate synthase (IspF) from Mycobacterium tuberculosis
            and Plasmodium falciparum
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3919)
  AUTHORS   Kaiser J, Rohdich F.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-FEB-2010) Chemistry, Food Chemistry, Grindelallee 117,
            Hamburg 20146, Germany
REFERENCE   3  (bases 1 to 3919)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..3919
                     /organism="Expression vector pCDS-At"
                     /mol_type="other DNA"
                     /db_xref="taxon:754449"
     promoter        10..54
                     /label=T5 promoter
                     /note="bacteriophage T5 promoter for E. coli RNA 
                     polymerase, with embedded lac operator"
     protein_bind    30..46
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     protein_bind    62..78
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             97..108
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             115..621
                     /codon_start=1
                     /product="2-methylerythritol 2,4-cyclodiphosphate synthase"
                     /label=2-methylerythritol 2,4-cyclodiphosphate synthase
                     /note="IspF; derived from Arabidopsis thaliana ispF gene; 
                     contains an N-terminal HisTag"
                     /protein_id="ADF28651.1"
                     /translation="MRGSHHHHHHGSRIGHGFDLHRLEPGYPLIIGGIVIPHDRGCEAH
                     SDGDVLLHCVVDAILGALGLPDIGQIFPDSDPKWKGAASSVFIKEAVRLMDEAGYEIGN
                     LDATLILQRPKISPHKETIRSNLSKLLGADPSVVNLKAKTHEKVDSLGENRSIAAHTVI
                     LLMKK"
     CDS             127..144
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     terminator      666..760
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             804..1463
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     terminator      1528..1614
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     misc_feature    1769..1909
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(2095..2683)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2854..3714)
                     /codon_start=1
                     /product="beta-lactamase"
                     /label=beta-lactamase
                     /protein_id="ADF28652.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(3715..3819)
                     /gene="bla"
                     /label=AmpR promoter