LOCUS       Exported                4741 bp ds-DNA     circular SYN 17-DEC-2018
DEFINITION  Mammalian expression vector pcDNAhRAIDD-E, complete sequence.
ACCESSION   LT727273
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4741)
  AUTHORS   De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman 
            Fonseca M, Vanhoucke M, Beyaert R.
  TITLE     BCCM/LMBP Plasmid collection
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4741)
  AUTHORS   De Schamphelaire W.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2017) BCCM/LMBP, Universiteit Gent, 
            Technologiepark 927, 9052, BELGIUM
REFERENCE   3  (bases 1 to 4741)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..4741
                     /organism="Mammalian expression vector pcDNAhRAIDD-E"
                     /mol_type="other DNA"
                     /note="BCCM/LMBP Plasmid collection (Ghent 
                     University,Belgium) accession number LMBP 3851."
                     /db_xref="taxon:1944943"
     rep_origin      25..160
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     rep_origin      complement(36..111)
                     /direction=LEFT
                     /label=SV40 ori
                     /note="SV40 ori"
     rep_origin      complement(262..374)
                     /direction=LEFT
                     /label=PY-O
                     /note="PY-O"
     polyA_signal    1006..1140
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     regulatory      complement(1066..1162)
                     /regulatory_class="terminator"
                     /note="polyA"
     regulatory      1066..1162
                     /regulatory_class="terminator"
                     /note="polyA"
     misc_feature    complement(1163..1561)
                     /label=3 UTR of SV40 small t-antigen
                     /note="3 UTR of SV40 small t-antigen"
     CDS             complement(1412..1432)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     misc_feature    complement(1562..1628)
                     /label=intron of SV40 small t-antigen
                     /note="intron of SV40 small t-antigen"
     intron          1562..1627
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             complement(1629..1703)
                     /codon_start=1
                     /note="unnamed protein product; part of SV40 small 
                     t-antigen"
                     /protein_id="SJL87961.1"
                     /translation="DLCEGTLLLWCDIIGQTTYRDLKL"
     promoter        1711..1729
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     regulatory      1711..1727
                     /regulatory_class="promoter"
                     /note="SP6"
     misc_feature    complement(1871..1909)
                     /label=E-tag
                     /note="E-tag"
     CDS             complement(1925..2521)
                     /codon_start=1
                     /note="unnamed protein product; hRAIDD"
                     /protein_id="SJL87962.1"
                     /translation="MEARDKQVLRSLRLELGAEVLVEGLVLQYLYQEGILTENHIQEIN
                     AQTTGLRKTMLLLDILPSRGPKAFDTFLDSLQEFPWVREKLKKAREEAMTDLPAGDRLT
                     GIPSHILNSSPSDRQINQLAQRLGPEWEPMVLSLGLSQTDIYRCKANHPHNVQSQVVEA
                     FIRWRQRFGKQATFQSLHNGLRAVEVDPSLLLHMLE"
     regulatory      2518..2527
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     promoter        complement(2554..2572)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      complement(2556..2572)
                     /regulatory_class="promoter"
                     /note="T7"
     regulatory      complement(2617..3200)
                     /regulatory_class="promoter"
                     /note="hCMV"
     promoter        complement(2617..2820)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     enhancer        2821..3200
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     misc_feature    complement(3355..3557)
                     /label=supF
                     /note="supF"
     tRNA            complement(3359..3538)
                     /product="E. coli amber-suppressor tyrosine tRNA"
                     /label=supF
     rep_origin      3681..4139
                     /label=M13-O
                     /note="M13-O"
     rep_origin      complement(4158..4390)
                     /direction=LEFT
                     /label=pMB1 ori
                     /note="pMB1 ori"