LOCUS       Exported                7063 bp ds-DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector pcDNA3.1+PA, complete sequence.
ACCESSION   EF550208
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7063)
  AUTHORS   Vahedi F, Taiebi Meibody N, KianiZadeh M, Mahmoudi M.
  TITLE     Construction of a Eukaryotic Plasmid Encoding Bacillus anthracis 
            Protective Antigen, a Candidate for DNA Vaccine
  JOURNAL   Iran J Immunol 2, 134-140 (2005)
REFERENCE   2  (bases 1 to 7063)
  AUTHORS   Vahedi F, Kyd J, Julla GM, Afzalaghaeei M, Kianizadeh M, Mahmoudi M.
  TITLE     A DNA vaccine candidate for B. anthracis immunization, pcDNA3.1+PA 
            plasmid, induce Th1/Th2 mixed responses and protection in mice
  JOURNAL   World J. Microbiol. Biotechnol. 24 (10), 2171-2178 (2008)
REFERENCE   3  (bases 1 to 7063)
  AUTHORS   Vahedi F, Mahmoudi M.
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2007) Immunology, Razi Vaccine and Serum 
            Institute, Ahmad Abad, Mashhad 91735, Iran
REFERENCE   4  (bases 1 to 7063)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..7063
                     /organism="Cloning vector pcDNA3.1+PA"
                     /mol_type="other DNA"
                     /db_xref="taxon:442152"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     promoter        863..881
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     gene            920..2605
                     /gene="pa"
                     /label=pa
                     /note="protective antigen fragment; derived from Bacillus 
                     anthracis isolate 34F2 plasmid pXO1"
     polyA_signal    2663..2887
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      2933..3361
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        3375..3704
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      3555..3690
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             3771..4565
                     /codon_start=1
                     /gene="aph(3)-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     polyA_signal    4739..4860
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4909..4925)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    4933..4949
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(4957..4987)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5002..5023
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(5311..5896)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(6067..6927)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(6928..7032)
                     /gene="bla"
                     /label=AmpR promoter