LOCUS       Exported                8223 bp ds-DNA     circular SYN 17-DEC-2018
DEFINITION  Expression vector pcDNA3-hFIX, complete sequence.
ACCESSION   DQ886588
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8223)
  AUTHORS   Hosseini SJ, Zomorodipour A, Jalal R, Sabouni F.
  TITLE     A recombinant plasmid for specific expression of human factor IX in 
            kertinocytes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8223)
  AUTHORS   Hosseini SJ, Zomorodipour A, Jalal R, Sabouni F.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-AUG-2006) Molecular Genetics, The National Institute 
            for Genetic Engineering and Biotechnology, Blvd. Pjoohesh. km-17 
            Tehran-karaj Highway, Tehran 14155-6343, Iran
REFERENCE   3  (bases 1 to 8223)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..8223
                     /organism="Expression vector pcDNA3-hFIX"
                     /mol_type="other DNA"
                     /db_xref="taxon:402769"
     CDS             complement(260..277)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     gene            2258..3670
                     /gene="FIX"
                     /label=FIX
                     /note="hFIX"
     CDS             2285..3670
                     /codon_start=1
                     /gene="FIX"
                     /product="coagulation factor IX"
                     /label=FIX
                     /note="derived from Homo sapiens; Christmas factor; 
                     Christmas-Eve factor; expression under control of Homo 
                     sapiens keratin-14 promoter elements"
                     /protein_id="ABI74450.1"
                     /translation="MQRVNMIMAESPGLITICLLGYLLSAECTVFLDHENANKILNRPK
                     RYNSGKLEEFVQGNLERECMEEKCSFGEAREVFENTERTTEFWKQYVDGDQCESNPCLN
                     GGSCKDDINSYECWCPFGFEGKNCELDVTCNIKNGRCEQFCKNSADNKVVCSCTEGYRL
                     AENQKSCEPAVPFPCGRVSVSQTSKLTRAETVFPDVDYVNSTEAETILDNITQSTQSFN
                     DFTRVVGGEDAKPGQFPWQVVLNGKVDAFCGGSIVNEKWIVTAAHCVETGVKITVVAGE
                     HNIEETEHTEQKRNVIRIIPHHNYNAAINKYNHDIALLELDEPLVLNSYVTPICIADKE
                     YTNIFLKFGSGYVSGWGRVFHKGRSALVLQYLRVPLVDRATCLRSTKFTIYNNMFCAGF
                     HEGGRDSCQGDSGGPHVTEVEGTSFLTGIISWGEECAMKGKYGIYTKVSRYVNWIKEKT
                     KLT"
     promoter        complement(3775..3793)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     polyA_signal    3819..4043
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      4089..4517
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        4531..4861
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4712..4847
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             4928..5722
                     /codon_start=1
                     /gene="neo"
                     /product="Neo"
                     /label=neo
                     /note="neomycin resistance"
                     /protein_id="ABI74451.1"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     gene            4928..5722
                     /gene="neo"
                     /label=neo
     polyA_signal    5896..6017
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6066..6082)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    6090..6106
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(6114..6144)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6159..6180
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(6468..7056)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(7227..8087)
                     /codon_start=1
                     /gene="amp"
                     /product="beta-lactamase"
                     /label=amp
                     /note="ampicillin resistance; Amp (Bla)"
                     /protein_id="ABI74452.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     gene            complement(7227..8087)
                     /gene="amp"
                     /label=amp
                     /note="bla"
     promoter        complement(8088..8192)
                     /gene="bla"
                     /label=AmpR promoter